Artificial substrate for researching chilo suppressalis oviposition selection behaviors and test method

Abstract

The invention discloses an artificial substrate for researching chilo suppressalis oviposition selection behaviors and a test method. The artificial substrate includes a support frame, an ovipositionsurface medium coats the exterior of the support frame, a tray is arranged at the bottom of the support frame, an open container is arranged inside the support frame, and two odor sources are placed inside the open container. By arranging the plant odor sources of different species in the artificial substrate or coating the artificial substrate with nylon nets with different mesh sizes, the chilosuppressalis oviposition selection behaviors on oryza of different species or different surface media are compared. By adopting the artificial substrate, not only can the same superficial area of theartificial substrate and uniform release of volatile matter be ensured, but also direct contact between female chilo suppressalis and plants is avoided, and therefore, oviposition stimulation effectsbetween different odor sources and different surface media can be compared specifically. The artificial substrate can not only meet requirements of egg collection during conventional artificial feeding but also provide bases for developing chilo suppressalis oviposition attractants or repellents and accordingly exploring new pest preventing and treating means.

Description

technical field [0001] The invention belongs to the field of biological research, and in particular relates to an artificial substrate and a test method for studying the egg-laying selection behavior of Chilo suppressalis. Background technique [0002] Chilo suppressalis is an important food crop pest. It is not only widely distributed in rice producing areas in my country, Southeast Asia and South Asia, but also is the dominant species of borer in most rice areas (Lu Yanhui et al. 2017). Due to the characteristics of borer damage of the insect, it is difficult to achieve the desired effect by using conventional control methods; and with the adjustment of the farming system and planting structure in rice areas in recent years and the unreasonable use of chemical pesticides, etc. The level of medicinal properties continues to increase, and the number of occurrences shows a clear upward trend (Dai Changgeng et al. 2018; Peng Shaobing et al. 2016; Zhang Yang et al. 2014). Thes...

AI Technical Summary

Problems solved by technology

Due to certain differences in the physical characteristics and contact compounds on the leaf surface of different species of host or non-host plants, the research results cannot correctly reflect the differences in volatile compounds of the compared plants, and cannot accurately explain that Chilo borer senses the host through the olfactory system. Plant volatiles, thus completing the whole process of host search, location and final acceptance

In addition, the egg masses laid by females in such studies are located on opaque plant leaves, and the egg masses need to be peeled off from the leaves before they can be observed and counted under a microscope, so it is often difficult to ensure the integrity of the egg masses during the peeling process

[0005] Although a few studies aimed at the olfactory perception signal of Chilo suppressalis and adopted bagging (Hao Lixia 2009), which avoided the direct contact between the tested females and plants, the non-woven gauze used as the egg-laying medium was soft and wrapped. After the plants cannot be fixed and formed, the volatiles released by the plants to the surface of the spawning medium are not uniform, and the comparability of the tested materials is not ideal

In addition, the oviposition device for adult Chilo suppressalis invented by Du Yuzhou et al. (2016) is mainly composed of an oviposition cylinder whose inner wall is covered with sulfuric acid paper and a disposable paper cup with rice seedlings inside. Seal with perforated sulfuric acid paper to provide an odor source that stimulates spawning. Compared with nylon nets, the number of holes on the surface of artificially perforated sulfuric acid paper is limited and unevenly distributed, so this invention is only used to meet the needs of collecting insects in artificial breeding. eggs, but cannot be used as an ideal test tool for bioassays

It can be seen that there are certain technical gaps in the experimental props and biological assay methods for studying the egg-laying selection behavior of Chilo suppressalis

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