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Preparation method and applications of support-free Janus monomolecular layer protein membrane material with adjustable membrane flux

A monolayer, protein membrane technology, applied in fermentation and other directions, can solve problems such as difficult to control membrane flux

Active Publication Date: 2019-09-03
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to accurately prepare a two-dimensional monolayer membrane material and solve the problem that the existing membrane flux is difficult to control, the present invention provides a preparation of a self-assembled two-dimensional unsupported Janus monolayer protein membrane material at the oil-water two-phase interface Method and its application, the monolayer protein membrane prepared by this method has good biocompatibility, can change the function of membrane flux independently according to the stimulation of ambient temperature, and has broad application in hemodialysis and sustained release delivery of drugs Application prospects

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  • Preparation method and applications of support-free Janus monomolecular layer protein membrane material with adjustable membrane flux
  • Preparation method and applications of support-free Janus monomolecular layer protein membrane material with adjustable membrane flux
  • Preparation method and applications of support-free Janus monomolecular layer protein membrane material with adjustable membrane flux

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specific Embodiment approach 1

[0029] Specific embodiment one: what this embodiment records is a kind of preparation method of unsupported Janus monolayer protein membrane material that membrane flux can be regulated, and the specific steps of described method are:

[0030] Step 1: Synthesize PNIPAAm, combine it with aminated bovine serum albumin (BSA-NH 2 ) coupling can prepare protein-polymer coupling body BSA-NH 2 / PNIPAAm;

[0031] Step 2: Preparation of aqueous phase solution: the protein-polymer coupling body BSA-NH prepared in step 1 2 / PNIPAAm is added into ultrapure water to prepare an aqueous phase solution;

[0032] Step 3: Prepare the oil phase solution: add the crosslinking agent into the oil phase solvent to prepare the oil phase solution;

[0033] Step 4: Add the oil phase solution in step 3 to the water phase solution in step 2, the oil and water phases are separated, and stand for 3 to 6 hours to make the protein-polymer coupling body BSA-NH 2 / PNIPAAm self-assembles into a monolayer pr...

specific Embodiment approach 2

[0038]Embodiment 2: In Embodiment 1, a method for preparing an unsupported Janus monolayer protein membrane material with adjustable membrane flux, in step 1, the molecular weight of the PNIPAAm is 2 kDa-200 kDa.

specific Embodiment approach 3

[0039] Specific embodiment three: the preparation method of a kind of unsupported Janus monolayer protein membrane material whose membrane flux can be regulated in specific embodiment one, in step two, the concentration of the aqueous phase solution is 0.04~1.25mg / mL.

[0040] Embodiment 4: The preparation method of a kind of unsupported Janus monolayer protein membrane material whose membrane flux can be regulated in Embodiment 1, in step 3, in the oil phase solution, the crosslinking agent and the oil phase The volume ratio of the solvent is 1:100-1000; the cross-linking agent is glutaraldehyde or polyethylene glycol-active ester (PEG-NHS), and the PEG-NHS includes different PEG chain lengths; the oil phase solvent One of n-octanol, isooctyl alcohol, benzene, toluene, lower hydrocarbon-substituted benzene, pentane, n-hexane, cyclohexane, chloroform, carbon tetrachloride, nitrobenzene, bromobenzene or chloroform.

[0041] Specific embodiment five: the preparation method of ...

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Abstract

The invention discloses a preparation method and applications of a monomolecular layer protein membrane material with adjustable membrane flux, and belongs to the technical field of biofilm preparation. The method comprises: preparing an aqueous phase: adding a protein-polymer conjugate BSA-NH2 / PNIPAAm to ultra-pure water to prepare an aqueous phase solution; preparing an oil phase: adding a cross-linking agent to an oil phase solvent to prepare an oil phase solution; and adding the obtained oil phase solution to the prepared aqueous phase solution, and standing for 3-6 h, such that the protein-polymer conjugate BSA-NH2 / PNIPAAm self-assembles into the monomolecular layer protein membrane at the oil-water two-phase solution interface. According to the present invention, the preparation of the two-dimensional support-free monomolecular layer protein membrane (thickness of 4-10 nm) can be achieved; the membrane material has a Janus structure (ie, the hydrophilicity and the hydrophobicityof the upper side and the lower side of the membrane are different); the membrane flux (the molecular weight of the permeable molecule) can be precisely controlled, and the oil-water two-phase catalytic reaction can be controlled by regulating the membrane flux through the temperature.

Description

technical field [0001] The invention belongs to the technical field of biofilm preparation, and in particular relates to a preparation method and application of an unsupported Janus monolayer protein film material with adjustable membrane flux. Background technique [0002] Two-dimensional unsupported monolayer membrane materials play a crucial role in the development of biomimetic simulation of natural biological membranes, biointerface catalysis, and artificial cells. Because most of the biological reactions are catalytic reactions of oil-water two-phase, it is difficult to accurately control the start and end of the reaction and the reaction rate. The development of two-dimensional unsupported monolayer membrane materials provides methods and approaches to solve this problem, mainly because two-dimensional membrane materials have good selectivity and permeability that can be adjusted, so they have the ability to control interfacial catalysis or two Possibility of phase-c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J5/18C08J3/24C08L33/24C08L89/00C12P7/20C12P7/52
CPCC08J5/18C08J3/24C12P7/52C12P7/20C08J2333/24C08J2389/00C08J2433/24C08J2489/00
Inventor 王磊孙照玲林幼萍刘小曼黄鑫
Owner HARBIN INST OF TECH
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