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Molecular genetic marker related to boar sperm motility and its application and acquisition method thereof

A molecular genetic marker and sperm motility technology, applied in the field of molecular markers, can solve the problems of high computational intensity, overestimation of marker effects, unreasonable significance threshold setting, etc., and achieve the effect of improving efficiency and sperm motility

Active Publication Date: 2019-09-03
广西贵港秀博基因科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such methods often face problems such as high computational intensity, overestimation of marker effects, and unreasonable setting of significance thresholds.

Method used

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  • Molecular genetic marker related to boar sperm motility and its application and acquisition method thereof
  • Molecular genetic marker related to boar sperm motility and its application and acquisition method thereof
  • Molecular genetic marker related to boar sperm motility and its application and acquisition method thereof

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Effect test

Embodiment 1

[0050] 1. Phenotype-pedigree data collection

[0051] The basic research population of this application is Duroc boars, all of which come from the boar station of Guangxi Xiubo Co., Ltd. The complete pedigree contains 12 generations of 5284 breeding pigs, of which 2693 boars recorded sperm motility traits from 2015 to 2018 Type data; sperm motility was obtained by analyzing fresh semen with the UltiMateTM CASA (Hamilton Thorne Inc., Beverly, MA, USA) system. A total of 143,114 semen trait observations (average 53 data per boar) were obtained for phenotype-genotype association analysis.

[0052] 2. Genotyping and quality control

[0053] The ear tissue samples or blood samples of 1733 boars were collected, the total DNA was extracted, and genotyping was performed using the GGP 50k SNP (GeneSeek, US) chip, and 50705 SNP markers covering the whole genome were obtained. According to the latest version of the pig reference genome (Sscrofa11.1), the physical positions of all SNP m...

Embodiment 2

[0085] According to the genetic results obtained by the above screening, it is shown that the molecular genetic marker related to the motility of boar sperm in the present application is located at the 8482542 nucleic acid site of the No. 17 chromosome of the pig, and this position is a C>T The mutation (Sscrofa10.2) corresponds to the 101st nucleic acid site of SEQ ID NO.1 in the nucleic acid sequence table.

Embodiment 3

[0087] Those skilled in the art can easily design primers for amplifying the molecular marker or probes for identifying the molecular marker based on the molecular genetic marker of the present invention, so as to be used for the detection of the genetic marker, such as obtaining the molecular marker by PCR amplification. The above molecular genetic markers, and then obtain the corresponding sequence by cloning and sequencing, or detect by Bsm-RFLP polymorphism. Therefore, the present invention also includes primers for amplifying said molecular genetic markers or probes for identifying said molecular genetic markers, and kits containing said primers or probes.

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Abstract

The invention relates to the technical field of molecular markers, in particular to a molecular genetic marker related to boar sperm motility and an application and an acquisition method thereof. Themolecular genetic marker related to boar sperm motility is located at the 8482542 bp position of chromosome 17 of pig, and is a mutation that C is greater than T; the molecular marker of the present application is obtained by single step genome-wide association study (wssGWAS); and the method is simple, efficient and fast.

Description

[0001] 【Technical field】 [0002] The invention relates to the technical field of molecular markers, in particular to a molecular genetic marker related to boar sperm motility and its application and acquisition method. [0003] 【Background technique】 [0004] With the development of the modern pig industry, the scale of pig farming is getting bigger and bigger, and the degree of intensification and standardization is also improving. my country's pig breeding has reached the first place in the world, with 50.6% of the world's fattened pigs and 44.23 million sows. Pork is also the food with the largest meat consumption in my country, so the quality and output of pork are directly related to the living standards of the people and affect the economic benefits of farmers. Two key factors in improving production efficiency are semen quality and sow fertility. [0005] There are many factors affecting reproductive efficiency, and the quality of boar semen is one of the important fa...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172
Inventor 赵云翔高宁朱琳张从林彭兴江威刘沁沅郑伟
Owner 广西贵港秀博基因科技股份有限公司
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