Device and method for improving gibberellin fermentation by vegetable oil

Abstract

The invention relates to the microbial field, in particular to a device and a method for improving gibberellin fermentation by vegetable oil. The device comprises a containing structure, a stirring structure and a driving structure, wherein an oil dripping mechanism is mounted in the containing structure; the oil dripping mechanism comprises a press block, a spring, a push rod, an oil filler, an oil outlet and an oil storage chamber, the oil filler is arranged on the outer side wall of the containing structure and communicates with the inside of the oil storage chamber, and the oil outlet is arranged at the bottom of the oil storage chamber; and the oil storage chamber is made of a rubber material, the push rod is arranged between the oil storage chamber and the containing structure, the spring is mounted on the outer side wall of the push rod, the press block is connected with one end of the push rod, and the top of the spherical press block props against bulges at bottoms of stirringblades. The device and a method for improving gibberellin fermentation by vegetable oil have the advantages of reducing foam produced during gibberellin fermentation and improving gibberellins fermentation efficiency.

Description

Technical field [0001] The invention relates to the field of microorganisms, in particular to a device and method for improving gibberellin fermentation by using vegetable oil. Background technique [0002] As an efficient and broad-spectrum plant growth hormone, Gibberellin has a wide range of applications in agroforestry production and beer fermentation. With the promotion of hybrid rice seed production technology and the continuous expansion of the fruit and vegetable planting industry, the commercial demand for gibberellin is also increasing, but the higher price of gibberellin restricts its further use. At present, GA3 is one of the most widely used plant growth hormones. With the deepening of research and market differentiation needs, its homologs GA4 and GA7 have received more and more attention. In view of the fact that there are no high-yielding GA4 production strains in China, and due to the limitation of fermentation technology, the production is a mixture of GA4+7, a...

AI Technical Summary

Problems solved by technology

Excessively high dissolved oxygen in the fermentation process will also lead to a decrease in the yield of gibberellin due to excessive bacterial growth and a large number of by-products.

[0004] At present, the amount of dissolved oxygen in the fermentation process is too low, which often causes changes in the metabolic process in the mycelium. For example, anaerobic respiration will produce a large amount of ethanol, acetic acid, etc., which is toxic to the cells; Autolysis, termination of fermentation, low titer

Therefore, in the fermentation process of gibberellin produced by Gibberella fujikura, sufficient dissolved oxygen is very important, and it is inconvenient to add vegetable oil in the traditional way

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