Detection method for glucose

A detection method and technology for glucose, applied in the field of medical detection, can solve the problems of low specificity, narrow detection range, low sensitivity, etc.

Pending Publication Date: 2019-09-13
JILIN UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a fast and simple

Method used

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  • Detection method for glucose
  • Detection method for glucose
  • Detection method for glucose

Examples

Experimental program
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Effect test

Embodiment 1

[0014] Embodiment 1 Synthesis of manganese dioxide nanoparticles

[0015] Add 0.1g BSA to 40mL 0.01mol L −1 Stir well in PBS buffer, then add 400µl 0.1MMnSO 4 solution, stirred at room temperature for 2 min; 400 µl 1M NaOH aqueous solution was added to the above mixture, and vigorously stirred for 7 h; after stirring, dialyzed in double distilled water for 48 h; Manganese nanoparticles MnO 2 NPs are prepared; the prepared MnO 2 NPs were stored in a 4°C refrigerator for later use.

Embodiment 2

[0016] Example 2 Construction of Glucose Detection System

[0017] 1. Build the system

[0018] First, 100 µl of glucose solutions of different concentrations (0.05, 0.158, 0.5, 1.58, 5, 15.8 mM) were mixed with 20 µl of glucose oxidase (GOD, 1 mg·ml -1 ) solutions were mixed, and the mixed solution was reacted on a water-bath shaker at 35°C for 8 min; then, 100 μl of the above-mentioned reacted solution was mixed with 100 μl NaOH (pH 12) 2 NPs (0.1mg·ml -1 ) and 50µl luminol (5×10- 5 M) working solution mixing; CL signal was measured with a full-band multifunctional microplate reader; all reactions were carried out in a light-tight white Greiner 96-well plate. With the logarithm of the glucose concentration as the abscissa and the CL signal as the ordinate, draw a standard curve.

[0019] 2. MnO 2 Verification experiment of signal amplification effect of NPs

[0020] In order to prove that MnO 2 The significant enhancement effect of NPs on the luminescent signal, refer...

Embodiment 3

[0023] Example 3 Sensitivity experiment of detection system

[0024] Under optimal working conditions, calculate the linear relationship between different concentrations of glucose and chemiluminescent intensity. The linear regression equation is y=5127.3x-8293.3 (where x is the logarithm of the glucose concentration based on 10, and y is the chemiluminescent intensity), and the correlation coefficient is 0.997; the linear range is 50μM~15.8mM ( Figure 8 ), and the detection limit was 44.7 μM (signal-to-noise ratio S / N=3).

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Abstract

The invention discloses a detection method for glucose and application of manganese dioxide nanoparticles (MnO<2>NPs) in the aspect of glucose detection. A glucose detection kit comprises a glucose oxidase solution, an alkaline aqueous solution, MnO<2>NPs and a luminol working solution. The detection method for the glucose comprises the steps of 1, mixing a to-be-detected sample with the glucose oxidase solution of which a concentration is 0.8-1.5mg/ml, and carrying out reaction for 6-10min at a temperature of 30-37 degrees centigrade; 2, taking 100mul of reacted solution, and mixing the reacted solution with 100mul of NaOH aqueous solution, 10mul of MnO<2>NPs with the concentration of 0.01-1.0mg.ml<-1> and 50mul of luminol working solution with the concentration of 0.1-10x10<-5>M; and 3,measuring a CL signal and computing glucose concentration according to a regression equation, wherein a linear range is 50muM-15.8mM, a detection limit is 44.7muM, and a detection result is consistentwith a measurement result of a commercial glucose meter well.

Description

technical field [0001] The invention belongs to the technical field of medical detection, and in particular relates to a glucose detection method. Background technique [0002] Glucose is one of the important components of the human body and an important source of energy. The glucose content in the blood must be maintained at a certain level to maintain the needs of various organs and tissues in the body, and changes in its concentration can reflect the health status of the human body. In recent years, with the improvement of living standards, diabetes has gradually become younger, and the incidence rate is increasing. The long-term high blood sugar in diabetes can lead to chronic damage and dysfunction of various tissues, especially the eyes, kidneys, heart, blood vessels, and nerves. Therefore, it is necessary to establish a blood glucose detection method with high sensitivity, good specificity and stable operation. [0003] At present, methods for detecting blood gluco...

Claims

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Application Information

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IPC IPC(8): G01N33/66G01N21/76
CPCG01N21/76G01N33/66
Inventor 宋秀玲司晓雪唐小飞赵超王娟徐坤李卉刘玉申张惠雯何施羽金明华
Owner JILIN UNIV
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