Application of rice dps1 gene and its encoded protein

A technology of DPS1 and rice, which is applied in the field of genetic engineering, can solve the problems of unexplored, reduce spikelet degeneration rate, and unresolved molecular mechanism of top spikelet degeneration, so as to achieve the effect of increasing rice yield and reducing incidence

Active Publication Date: 2021-03-16
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] So far, although several top spikelet degeneration-related genes have been cloned in rice, the molecular mechanism of top spikelet degeneration has not yet been clearly resolved, and a more complete and refined molecular regulatory network for rice top spikelet degeneration needs to be constructed.
In addition, genes that can be effectively applied to reduce the rate of spikelet degeneration have not been discovered

Method used

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  • Application of rice dps1 gene and its encoded protein
  • Application of rice dps1 gene and its encoded protein
  • Application of rice dps1 gene and its encoded protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The acquisition and phenotype analysis of embodiment 1 mutant

[0041] The japonica rice Shengdao 16 was mutagenized with ethyl methanesulfonate (EMS), and a panicle tip degenerated mutant dps1 (degenerated panicle and spikelet 1, dps1) was screened ( figure 1 A). In the early stage of growth and development, the vegetative growth and reproductive growth of the dps1 mutant were normal, and there was no obvious difference compared with the wild type, and continued until the young panicle developed to about 10 cm ( figure 1 the B, figure 1 C); when the young ear reached about 12cm, the spikelet at the top of the mutant ear was pale white, stagnated, and obvious degeneration occurred ( figure 1 the D, figure 1 E); with the further development of rice spikelets, the degenerated spikelets at the top continued to increase, and this degeneration phenomenon became more obvious when the spikelets reached their final length before anthesis, manifested as shriveled necrosis ( ...

Embodiment 2

[0044] The acquisition of embodiment 2 rice DPS1 gene

[0045] Crossing the dps1 mutant with the phenotypically normal indica rice variety Huanghuazhan, F 1 Behaved normally, in F 2 In the generation segregation population, the segregation ratio of normal individual plants and mutant individual plants was 3:1, which indicated that the mutant traits were controlled by a pair of recessive genes. with F 2 The 30 mutants were used as materials, and the candidate gene was located between the Indel markers M1 and M7 of the fifth chromosome by using 170 Indel markers evenly distributed on the 12 chromosomes of rice. In order to fine-map the gene, the F 2 The population was expanded to 472 mutant individuals, and the candidate genes were located within the 28.85kb range between INDEL markers M3 and M5 ( figure 2 A). This interval contains 6 open reading frames, among which the gene sequence numbered Os05g0395300, its gene function is related to the phenotype, so the full-length ...

Embodiment 3

[0048] Example 3 pCAMBIA1305.1:: DPS1 vector transformation of rice dps1 mutant

[0049] In order to carry out the functional complementation experiment, the DPS1 gene functional complementation vector driven by its own promoter and the overexpression vector driven by the rice ACTIN1 promoter were respectively constructed. The DPS1 gene functional complementation vector is driven by the gene's own promoter. The 2011 bp before the translation initiation site ATG is selected as the gene promoter, and the genomic DNA is used as the template for PCR amplification. The amplification primers used are DPS1-gDNA shown in Table 2. -C. The 5' end introduces an EcoRI site and the 3' end introduces a SpeI site, and the PCR product is 9685bp long (sequence shown in SEQ ID NO.1), and finally its own promoter together with the entire genomic DNA (shown in SEQ ID NO.1 sequence) were cloned into the pCAMBIA1305.1 vector together to form a complementary vector driven by its own promoter ( im...

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Abstract

The invention provides a rice DPS1 gene and encoded protein and application thereof. The sequence of the rice DPS1 gene is shown in the SEQ ID No.2, and the sequence of the encoded protein is shown in the SEQ ID No.1. It is found that the top spikelet of the mutant of the rice DPS1 gene is dry up and necrotic before flowering, and the middle spikelet closely adjacent to the top degenerated spikelet is sterile, which results in a significant decrease in the seed setting rate and the grain number per spike. The wild DPS1 gene is transformed into the rice DPS1 mutant, the degraded phenotype of the mutant spike top can be recovered, the result suggests that the DPS1 gene directly controls degeneration of the rice spike top by regulating programmed cell death, and the rice DPS1 gene can be applied to the directional design of rice spike types and used as an important genetic resource, is helpful to develop molecular markers in molecular breeding practice for conducting early identification and elimination on spike top degradation, and has important guiding significance for improving the rice yield.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and specifically relates to rice DPS1 gene, its encoded protein and application. Background technique [0002] Rice is one of the most important food crops in my country and even in the world. In my country, more than 60% of the population takes rice as a staple food. At the same time, with the acceleration of urbanization, the reduction of arable land and the application of bio-energy, the impact on food Demand is also increasing. Therefore, increasing rice production is of great significance to solving the increasingly serious food problem. Rice panicle yield is the most basic unit of rice yield. Larger panicle and more grains have always been one of the important goals of rice breeding in the world. However, the degeneration of the top of the ear is a relatively common biological phenomenon in rice breeding and field production, resulting in a decrease in the volume of the panicle and a sig...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/8261
Inventor 李学勇房静静赛义德·阿德尔·扎法尔赵金凤袁守江
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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