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ADSL gene and method of ADSL gene as genetic marker for inosine monophosphate content of Xupu geese

A technology of goinosinic acid and genetic markers, which is applied in the field of determination of polymorphisms in the ADSL gene, can solve the problems that genetic markers have not been reported yet, and achieve significant application value and economic benefits, as well as the effect of fresh muscle quality

Active Publication Date: 2019-09-17
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The research on DNA genetic markers, genetic diversity and related functional genes of Xupu goose is still in its infancy, and the genetic markers related to IMP in Xupu goose have not been reported yet.

Method used

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  • ADSL gene and method of ADSL gene as genetic marker for inosine monophosphate content of Xupu geese
  • ADSL gene and method of ADSL gene as genetic marker for inosine monophosphate content of Xupu geese
  • ADSL gene and method of ADSL gene as genetic marker for inosine monophosphate content of Xupu geese

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 SNP screening of Xupu goose pectoralis inosinic acid-related genes

[0037] Sixty healthy Xupu geese of the same age were selected for the filling-feeding experiment. After 28 days of slaughter, the inosinic acid content in breast muscle was detected by high performance liquid chromatography, and the genomic DNA was extracted from samples with high inosinic acid content and low inosinic acid content.

[0038] According to the goose ADSL gene sequence in GenBank, Primer5.0 was used to design primers covering the entire sequence of exon 9 to exon 10 of the gene. The primer sequence is:

[0039] Forward primer: 5’—CACCCATAAAGGGAACAG—3’

[0040] Reverse primer: 5'—AGCACAGACAGCCAGATT—3'

[0041] Primers were synthesized by Hunan Qingke Biotechnology Co., Ltd. The genomic DNA of Xupu goose was amplified with the above primers respectively.

[0042] PCR: 20 μl reaction system: 12 μl of 2×Master mix, 0.8 μl each of upstream and downstream primers (10 μmol / L), 1 μl...

Embodiment 2

[0047] Example 2 Verification of the SNPs related to the inosinic acid content of Xupu goose pectoralis in production practice

[0048] Twenty healthy Xupu geese aged 80 days and under the same feeding and management conditions were selected for the experiment. After 28 days of feeding, all the tested Xupu geese were slaughtered to detect the content of inosinic acid in breast muscle.

[0049] Genomic DNA extracted from 20 Xupu geese pectoralis tissue samples was amplified using the primers in Example 1, and the SNPs were detected according to the method in Example 1.

[0050] Table 1 PCR reaction system

[0051]

[0052] The PCR cycling parameters are as follows:

[0053] Pre-denaturation at 95°C for 5min; denaturation at 95°C for 30s, annealing at 57.6°C for 30s, extension at 72°C for 45s, a total of 35 cycles; extension at 72°C for 10min, and finally storage at 4°C.

[0054] Genotype frequency analysis of ADSL gene SNP locus

[0055] The genotype frequency and allele...

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Abstract

The invention discloses an adenylosuccinate lyase (ADSL) gene and application of the ADSL gene as a genetic marker for the inosine monophosphate content of Xupu geese. The inosine monophosphate content in the pectoral muscle of the Xupu geese is measured, the polymorphism of the ADSL gene of the Xupu geese is screened, and then association analysis is conducted on the polymorphism of the ADSL gene and the inosine monophosphate character of the pectoral muscle, so that it is found that the ADSL gene of the Xupu geese is shown as a sequence in which a G / T mutation exists at a 146 bp locus, and the inosine monophosphate content of the pectoral muscle of the GG and TT genotype Xupu geese is significantly higher than that of the GT genotype. The molecular marker is provided for marker-assisted selection of the inosine monophosphate characters of the pectoral muscles of the Xupu geese.

Description

technical field [0001] The invention belongs to the field of poultry molecular biotechnology and breeding, and is mainly used for determining the genetic markers related to the content of inosinic acid in the muscle of Xupu goose. The presence of a single nucleotide polymorphism (Singlenucleotide polymorphism, abbreviated as SNP) in the adenosine succinate lyase gene associated with inosinic acidity. [0002] technical background [0003] As a local breed resource, Xupu goose has delicious meat and rich in essential nutrients for human body, which is very beneficial to human health. Goose meat is rich in nutrients, rich in various amino acids, proteins, multivitamins, sugars, and trace elements necessary for the human body, and has a low fat content and high unsaturated fatty acid content, which is easily digested and absorbed by the human body. In 2002, the Food and Agriculture Organization of the United Nations listed goose meat as one of the green foods that should be dev...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/60
CPCC12Q1/6888C12N9/88C12Y403/02002C12Q2600/124C12Q2600/156
Inventor 曲湘勇刘耀文郭松长贺长青
Owner HUNAN AGRICULTURAL UNIV