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Curdlan gum purification method and application

A purification method and technology of alkaline solution, applied in the field of industrialized production of keratin, can solve the problems of unsatisfactory purity and gel strength, ineffective enzyme preparation, poor separation effect, etc., to achieve comparable appearance transparency and reduce processing capacity The effect of reducing production equipment cost and viscosity reduction time

Active Publication Date: 2019-09-20
SHANDONG FOOD & FERMENT IND RES & DESIGN INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported that although the free bacteria in the fermentation broth can be degraded by adding protease, etc., in practice, it is found that a large number of bacteria cells are wrapped in the inner layer of curdlan, forming tiny insoluble particles, and the enzyme preparation cannot work. There are still a large number of bacteria in the solution, and the separation effect is not good
It is difficult to purify by physical separation, and the purified curdlan is not ideal in terms of purity and gel strength

Method used

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  • Curdlan gum purification method and application
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  • Curdlan gum purification method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0039] A high-efficiency sterilization method for microbial polysaccharide curdlan gum fermentation broth, the specific process steps are as follows:

[0040] (1) Dilute alkali solution

[0041] Add curdlan gum fermentation liquid in sodium hydroxide solution, constantly stir and make it dissolve completely, control the concentration of curdlan gum in the solution to be 1.6% (w / v), [OH - ] The concentration is 0.35mol / L. After the dissolution is completed, the initial viscosity of the solution is 1500mPa.s.

[0042] (2) High shear quickly reduces viscosity

[0043] After the dilution and alkali dissolution is completed, the curdlan gum solution is treated at a shear rate of 5000r / min for 6 minutes to reduce the viscosity rapidly to facilitate subsequent filtration and sterilization. After the treatment, the viscosity of the curdlan gum solution drops to 31mPa.s.

[0044] (3) Sterilization by filtration

[0045] The curdlan alkali solution whose viscosity is reduced in step...

Embodiment 2

[0055] A high-efficiency sterilization method for microbial polysaccharide curdlan gum fermentation broth, the specific process steps are as follows:

[0056] (1) Dilute alkali solution

[0057] Add curdlan gum fermentation liquid in sodium hydroxide solution, constantly stir and make it dissolve completely, control the concentration of curdlan gum in the solution to be 2.0% (w / v), [OH - ] The concentration is 0.50mol / L. After the dissolution is completed, the initial viscosity of the solution is 2300mPa.s.

[0058] (2) High shear quickly reduces viscosity

[0059] After the dilution and alkali dissolution is completed, the curdlan gum solution is treated at a shear rate of 7000r / min for 8 minutes to reduce its viscosity rapidly, so as to facilitate subsequent filtration and sterilization. After treatment, the viscosity of curdlan gum solution drops to 52mPa.s.

[0060] (3) Sterilization by filtration

[0061] The curdlan alkali solution whose viscosity is reduced in step ...

Embodiment 3

[0071] A kind of high-efficiency sterilization method of microbial polysaccharide curdlan gum crude product, its specific process steps are as follows:

[0072] (1) Dilute alkali solution

[0073] Add the aqueous suspension of curdlan thick product in the sodium hydroxide solution, constantly stir and make it dissolve completely, control the concentration of curdlan in the solution to be 2.3% (w / v), [OH - ] The concentration is 0.60mol / L. After the dissolution is completed, the initial viscosity of the solution is 2800mPa.s.

[0074] (2) High shear quickly reduces viscosity

[0075] After the dilution and alkali dissolution, the curdlan solution was treated at a shear rate of 8000r / min for 10 minutes to reduce its viscosity rapidly to facilitate subsequent filtration and sterilization. After treatment, the viscosity of the curdlan solution dropped to 66mPa.s.

[0076] (3) Sterilization by filtration

[0077] The curdlan alkali solution whose viscosity is reduced in step (2...

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Abstract

The invention belongs to the technical field of industrial production of curdlan gum, and particularly relates to a curdlan gum purification method and application, aiming to solve the problems of microbial polysaccharide curdlan gum in the prior art, including low sterilizing efficiency and poor sterilizing effect of fermentation liquid, low product purity, low gel transparency, high ion concentration, and low strength of formed gum (based on GB28304). According to the high-efficiency curdlan gum fermentation liquid sterilization method, the curdlan gum fermentation liquid or crude curdlan gum products are dissolved in alkali liquor, the viscosity is quickly reduced through high shear, a filter is used for efficiently filtering out bacteria and insoluble impurities, acids are added to supernatant for neutralization and sedimentation to obtain curdlan gum, and the curdlan gum is subjected to filtration and concentration, wash desalting, ethyl alcohol sedimentation and drying to obtain a high-quality curdlan gum product. The method is simple and convenient to operate, economical, and applicable to large-scale industrial production of the high-quality curdlan gum.

Description

technical field [0001] The disclosure belongs to the technical field of curdlan gum industrial production, in particular to a microbial polysaccharide curdlan gum efficient sterilization, purification method and application. Background technique [0002] The information disclosed in this Background section is only intended to increase the understanding of the general background of the disclosure, and is not necessarily to be taken as an acknowledgment or any form of suggestion that the information constitutes prior art that is already known to those skilled in the art. [0003] Microbial polysaccharide Curdlan gum (Curdlan), also known as thermal gel, belongs to the extracellular secretion of microorganisms. It is composed of about 300-500 glucose residues linked by β-1,3-D-glycosidic bonds, and has no branches. Homogeneous Polysaccharide Polymer. Curdlan is widely used in the food industry as a food additive because it is safe and non-toxic to the human body and has good g...

Claims

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Application Information

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IPC IPC(8): C08B37/02
CPCC08B37/0024C08B37/0003
Inventor 董学前张永刚王伟张艳敏
Owner SHANDONG FOOD & FERMENT IND RES & DESIGN INST
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