Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of expression infectious laryngotracheitis virus gb gene, its recombinant fowlpox virus and its construction method and application

A technology for laryngotracheitis virus and fowl pox virus, which is applied in the directions of virus, application, virus peptide, etc., can solve the problems of indistinguishability between wild virus strains and vaccine strains, economic loss of poultry industry, and strong virulence of live attenuated vaccines. , to achieve the effect of overcoming virulence reversion and latent infection, large market economic potential, and significant immune protection level

Active Publication Date: 2020-09-08
YANGZHOU UNIV
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disease has the characteristics of rapid spread, high mortality, and can cause a rapid decline in the egg production rate of laying hens, causing huge economic losses to the poultry industry
At present, the disease is mainly prevented by attenuated live vaccines at home and abroad, but the attenuated live vaccines have the risk of returning to strong virulence. Some attenuated live vaccines can also cause chickens to become ill, causing the outbreak and prevalence of ILT. Many defects such as indistinguishability from vaccine strains

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of expression infectious laryngotracheitis virus gb gene, its recombinant fowlpox virus and its construction method and application
  • A kind of expression infectious laryngotracheitis virus gb gene, its recombinant fowlpox virus and its construction method and application
  • A kind of expression infectious laryngotracheitis virus gb gene, its recombinant fowlpox virus and its construction method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Construction of transfer vector

[0050] The fowlpox virus transfer vector p12LS contains two fowlpox virus replication non-essential region genes, MCS and promoter Ps, and can insert the target gene in the transfer vector into the fowlpox virus by using the principle of homologous recombination.

[0051] The mutated plasmid T3-gB was electrophoresed with BamH I and Xho I to recover the target fragment of 2.7 kb, which was ligated with the vector plasmid p12LS cut with BamH I and SalI, and the plasmids, BamH I and Sma I were extracted For double enzyme digestion identification, take the plasmid with the correct band size and send it to Nanjing GenScript Company for sequencing. The positive plasmid is the transfer vector p12LS-gB, and the BamH I+Sma I double enzyme digestion identification result of p12LS-gB is as follows figure 2 Shown, wherein, M: 1kbplus DNAladder; 1: p12LS-gB digested by BamH I+Sma I.

[0052] Transfection and Purification of Recombinant Fowlpox Vi...

Embodiment 2

[0084] recombinant fowl pox virus rFPV 282E4 -gB immune potency

[0085] The 28-day-old SPF chickens were randomly divided into 10 groups, according to 10 5 The dose of PFU / bird was immunized with rFPV respectively 282E4 -gB, fowlpox parental strain wt-FPV 282E4 At the same time, a control group of commercialized ILTV recombinant fowlpox virus genetically engineered vaccine was established, and the dose was 10 5 PFU / rat; PBS control group, the dose was 0.2mL / rat. 21 days after immunization, use 10 5 EID 50 The ILTV wild strain Gallidherpesvirus 1strain I19 per chicken was used for throat challenge, and the morbidity and mortality of each vaccinated chicken were observed, and throat swabs were collected to determine the detoxification situation.

[0086] (1) Clinical scores, morbidity and mortality after challenge

[0087] According to the clinical symptoms of each group of chickens after observing the challenge, carry out clinical scoring, scoring standard: normal=0 poi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a gB gene expressing infectious laryngotracheitis virus, its recombinant fowlpox virus, its construction method and application. The nucleotide sequence of the gB gene of the infectious laryngotracheitis virus of the present invention is shown in SEQ ID NO.1. Compared with the commercialized recombinant fowlpox genetically engineered vaccine, the recombinant fowlpox virus constructed by utilizing the gB gene of infectious laryngotracheitis virus described in the present invention has a more significant immune protection level, and can overcome the toxicity of traditional attenuated live vaccines. Defects such as force reversion and latent infection.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a gB gene expressing infectious laryngotracheitis virus, its recombinant fowlpox virus and its construction method and application. Background technique [0002] Infectious laryngotracheitis (ILT) is an acute respiratory infectious disease caused by infectious laryngotracheitis virus (ILTV). Endemic. The disease has the characteristics of rapid spread, high mortality, and can cause a rapid decline in the egg production rate of laying hens, which has caused huge economic losses to the poultry industry. At present, the disease is mainly prevented by attenuated live vaccines at home and abroad, but the attenuated live vaccines have the risk of returning to strong virulence. Some attenuated live vaccines can also cause chickens to become ill, causing the outbreak and prevalence of ILT. Many defects such as being difficult to distinguish from vaccine strains. Therefore, it is necessary...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/38C07K14/03C12N7/01C12N15/863A61K39/235A61P31/20
CPCA61K39/12A61K2039/53A61K2039/552A61P31/20C07K14/005C12N7/00C12N15/86C12N2710/16022C12N2710/16034C12N2710/24021C12N2710/24034C12N2710/24043
Inventor 彭大新他蕾陈素娟秦涛
Owner YANGZHOU UNIV