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Intelligent responding type genetic carrier delivery system, and preparation method and application thereof

A gene carrier and intelligent technology, which is applied in the field of intelligent responsive gene carrier delivery system and its preparation, can solve the problems affecting carrier transmission performance and achieve broad application prospects and high transfection efficiency

Pending Publication Date: 2019-09-24
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, for the in vivo application of polycationic gene carriers, since blood contains many negatively charged proteins, it is easy to have electrostatic interactions with polycationic gene carriers, causing the aggregation of carrier / nucleic acid complex particles, which in turn affects the transport performance of the carrier.

Method used

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  • Intelligent responding type genetic carrier delivery system, and preparation method and application thereof
  • Intelligent responding type genetic carrier delivery system, and preparation method and application thereof
  • Intelligent responding type genetic carrier delivery system, and preparation method and application thereof

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preparation example Construction

[0049] The present invention also provides a preparation method of the antitumor drug of the present invention, comprising:

[0050] 1) Compounding the polycation in the intelligent responsive gene carrier delivery system with the drug to obtain the polycation drug complex;

[0051] 2) Mixing the thioketal-modified dextran and the polycation drug complex to obtain an antitumor drug.

[0052] According to the present invention, the present invention first compounded the polycation and the drug in the intelligent responsive gene carrier delivery system to obtain the polycation drug complex; then mixed the thioketal-modified dextran with the polycation drug complex to obtain Antineoplastic drugs; wherein, the present invention has no special requirements on the method of compounding or mixing, and those skilled in the art can select a suitable compounding or mixing method according to common knowledge in the art; wherein, the polycation of the intelligent responsive gene carrier ...

Embodiment 1

[0056] 1. Synthesis of DSDPA-Dex

[0057] Taking the Dex of molecular weight 20000 as example, anhydrous mercaptopropionic acid and anhydrous acetone mixture are filled with dry hydrogen chloride, react at room temperature, after the completion of the reaction, cool down under ice-salt mixture conditions until crystallization is complete. The obtained crystals were filtered, washed with n-hexane and ice water, and the obtained product was freeze-dried to obtain DSDPA.

[0058] Then add C 8 h 17 N 3 Under the condition of HCl and DMAP, according to the molar ratio of DSDPA and Dex in Table 1, mix respectively, obtain different proportions of DSDPA and Dex to obtain mixtures, and above-mentioned mixtures are dissolved in DMSO solution respectively, carry out reaction. The reacted mixture was then dialyzed against deionized water. Finally, the dialyzed product was freeze-dried to obtain thioketal-modified dextran (DSDPA-Dex).

[0059] Table 1 is the feed ratio corresponding ...

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Abstract

The invention provides an intelligent responding type genetic carrier delivery system, and a preparation method and application thereof. The intelligent responding type genetic carrier delivery system comprises a kernel and a shell coated on the kernel, wherein the shell is thioketal modified glucan, and the mole ratio of thioketal to glucan in the thioketal modified glucan is (1-1200) to 1; and the kernel is polycation. Through the result, the inventor finds that the intelligent responding type genetic carrier delivery system provided by the invention has lower cytotoxicity and excellent H2O2 responsiveness, and has high-efficiency transfection efficiency at a tumor position; and a mediate therapeutic gene of the constructed intelligent responding type genetic carrier delivery system has significant antitumor effect, namely that the intelligent responding type genetic carrier delivery system provided by the invention has wide application prospect in the field of gene therapy and tumor resistance.

Description

technical field [0001] The invention relates to the technical field of biomedical new materials, in particular to an intelligent responsive gene carrier delivery system and its preparation method and application. Background technique [0002] Cancer, as a malignant disease, threatens human life and health. In the treatment of cancer, gene therapy, as a brand-new treatment method, has attracted widespread attention. The key to gene therapy is efficient gene carrier. At present, gene vectors are mainly divided into viral gene vectors and non-viral gene vectors. Viral gene vectors have high immunogenicity, so their clinical application is limited. Among non-viral gene carriers, polycationic gene carriers have attracted great attention due to their unique properties. Generally, positively charged polycations and negatively charged nucleic acids form complex particles through electrostatic interactions to carry out gene loading and transport [see Fang, H.P.; Guo, Z.P.; Lin, L...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K47/36A61K47/34A61P35/00C08B37/02
CPCA61K48/0041A61K48/005A61K47/36A61K47/34A61P35/00C08B37/0009
Inventor 田华雨方华攀林琳陈杰陈学思
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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