Check patentability & draft patents in minutes with Patsnap Eureka AI!

Nucleic acid drug delivery system based on recombinant ribosomal protein as well as preparation method and application of nucleic acid drug delivery system

A ribosomal protein and nucleic acid drug technology, which can be used in pharmaceutical formulations, medical preparations with non-active ingredients, and medical preparations containing active ingredients, etc., can solve problems such as limited protein expression efficiency, and achieve good transfection efficiency. Good application prospect and easy biodegradation effect

Pending Publication Date: 2022-05-10
南京凯玛生物科技有限公司
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the efficiency of protein expression from mRNA-protamine particles is limited due to the tight binding of protamine and mRNA

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nucleic acid drug delivery system based on recombinant ribosomal protein as well as preparation method and application of nucleic acid drug delivery system
  • Nucleic acid drug delivery system based on recombinant ribosomal protein as well as preparation method and application of nucleic acid drug delivery system
  • Nucleic acid drug delivery system based on recombinant ribosomal protein as well as preparation method and application of nucleic acid drug delivery system

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0038] In one embodiment of the present invention, a method for preparing the above-mentioned recombinant ribosomal protein-based nucleic acid drug delivery system is provided, and the specific steps of the preparation method are as follows:

[0039](1) Preparation of poly-L-lysine derivative polymer (APP):

[0040] Poly-L-lysine and cis-aconitic anhydride were reacted for 6-10 hours at a pH of 9.0, the reaction solution was transferred to a dialysis bag, dialyzed in an aqueous solution with a pH of 8-9 for 22-26 hours, and then freeze-dried to obtain cis-aconitic anhydride. Poly-L-lysine AA-PLL grafted with aconitic acid;

[0041] React AA-PLL and NHS-PEG-MAL at pH 7.4 for 6-10 hours, dialyze and freeze-dry to obtain AA-PLL-MAL; graft RGD polypeptide on AA-PLL-MAL, and mix the two in an appropriate amount at pH React for 6-10 hours under the condition of 7.0 in an inert environment, and the product is obtained by dialysis and lyophilization to obtain maleimide-modified AA-PL...

Embodiment 1

[0049] Synthesis of Multifunctional Poly-L-Lysine Derivatives (APP)

[0050] 1. Synthesis of poly-L-lysine-AA-PLL grafted with cis-aconitic acid

[0051] Accurately weigh 5.0mg of poly-L-lysine and dissolve in 5ml of PBS with pH 9.0, then add precisely weighed 4.2mg of cis-aconitic anhydride to dissolve, and dissolve with 1.0M NaOH to adjust the pH of the mixed solution to about 9.0 , reacted at room temperature for 8 hours under electromagnetic stirring, transferred the reaction mixture to a dialysis bag with a molecular weight cut-off of 3500Da after leak detection in advance, dialyzed in an aqueous solution with a pH of 8-9 adjusted by NaOH for 24 hours, and then freeze-dried. Whether the target product is synthesized.

[0052] 2. Synthesis of AA-PLL-AA-PP-MAL modified by maleimide

[0053] Accurately weigh 9.0 mg of the above product AA-PLL and dissolve it in 5 ml of PBS with pH 7.4, add 26.0 mg of NHS-PEG-MAL into the AA-PLL solution, and react at room temperature for 8...

Embodiment 2

[0057] Poly-L-lysine-derived polymer-modified ribosomal protein-enriched mRNA delivery system—preparation of mFluc@RP / APP nanocomplex

[0058] Mix a series of ribosomal proteins RPL28, RPL32, RPL27, RPS17, RPL22 for screening with luciferase mRNA (the mass ratio of ribosomal protein to mRNA is 1, 2, 4, 8, 16 respectively), pipette Gently pipette for 10s, and incubate at room temperature for 30 minutes to promote the full combination of cationic protein and mRNA to form mFluc@RP complex. After centrifugation and resuspension, add a certain amount of poly-L-lysine derivative polymer (APP: protein 40:1 mass ratio), blow gently with a pipette gun for 10 seconds, incubate at room temperature for 30 minutes, and centrifuge and resuspend to obtain the prepared mFluc@RP / APP nanocomplex, which is an mRNA delivery system based on cationic ribosomal protein.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Mwcoaaaaaaaaaa
Login to View More

Abstract

The invention relates to the technical field of biological medicine, in particular to a nucleic acid drug delivery system based on recombinant ribosomal protein and a preparation method and application thereof.The delivery system comprises endogenous cationic ribosomal protein, a delivered nucleic acid drug and an outer layer modified polymer promoting release of the nucleic acid drug in lysosome, the endogenous cation ribosomal protein is selected from one of a 60S ribosomal protein L28, a 60S ribosomal protein L32, a 60S ribosomal protein L27, a 60S ribosomal protein S17 and a 60S ribosomal protein L22; preferably, 60S ribosomal protein L32, and the outer layer modified polymer for promoting the release of the nucleic acid medicine in the lysosome is a poly L-lysine derivative polymer. According to the present invention, the compressed nucleic acid drug can be effectively delivered into the cell, the transfection efficiency is good, the safety problem of the viral vector is avoided by the material, and the material has characteristics of low cytotoxicity, easy biodegradation and the like, and has good application prospects in the research of the nucleic acid drugs such as mRNA, DNA, siRNA, miRNA and the like.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a nucleic acid drug delivery system based on recombinant ribosomal protein and its preparation method and application. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] In recent years, the field of gene therapy has developed rapidly. Nucleic acid molecules, as a category of biopharmaceuticals that have attracted much attention and research, can achieve the goal of passive or active intervention and regulation of diseases. Patients provided innovative treatments. Among them, taking mRNA in nucleic acid molecules as an example, mRNA therapy uses mRNA pharmaceutical preparati...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K9/50A61K47/34A61K31/713A61K47/64A61K48/00
CPCA61K47/64A61K31/713A61K9/5031A61K48/0041A61K48/0025
Inventor 姜新义张蕊张晶陈晨张盛昌
Owner 南京凯玛生物科技有限公司
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com