Method for extracting phycocyanin from cyanobacteria
A phycocyanin and extraction method technology, applied in the field of protein extraction, can solve the problems of difficult industrialization, unsatisfactory yield, purity, and cost, and achieve the effects of short extraction cycle, low price and good stability
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Embodiment 1
[0033] A method for extracting phycocyanin in cyanobacteria, comprising the steps of:
[0034] (1) Wall breaking: mix blue-green algae and table salt at a mass ratio of 1:0.2, place in vacuum freezer until the temperature is minus 20°C, grind to 80 mesh, then heat to a temperature of 30-35°C, then add blue-green algae 2 times the mass of the isothermal extract was soaked at a constant temperature for 20 minutes; the extract was composed of the following parts by mass: 8 parts of sodium citrate, 17 parts of sodium lauryl sulfate, 6 parts of sodium tartrate, 12 parts of sodium dihydrogen phosphate, 90 parts parts deionized water;
[0035] (2) Coarse filtration: After filtering the broken wall product with a 0.5 μm microfiltration membrane, add oil and deionized water in turn to mix, and remove the oil through water and oil separation, and then filter with a 0.5 μm microfiltration membrane; The dosage is 20% of the mass of cyanobacteria, and the mixing time is 10min; the dosage ...
Embodiment 2
[0041] A method for extracting phycocyanin in cyanobacteria, comprising the steps of:
[0042] (1) Wall breaking: mix blue-green algae and table salt at a mass ratio of 1:0.1, put them in a vacuum freezer until the temperature is minus 10°C, grind to 50 meshes, then heat to a temperature of 30-35°C, and then add blue-green algae 1.5 times the mass of the isothermal extract is soaked at a constant temperature for 15-20 minutes; the extract is composed of the following parts by mass: 5 parts of sodium citrate, 13 parts of sodium lauryl sulfate, 2 parts of sodium tartrate, and 10 parts of sodium dihydrogen phosphate , 80 parts of deionized water;
[0043] (2) Coarse filtration: After filtering the broken wall product with a 1 μm microfiltration membrane, add oil and deionized water in turn to mix, and remove the oil through water and oil separation, and then filter with a 1 μm microfiltration membrane; the amount of oil The mass of cyanobacteria is 15%, and the mixing time is 5 ...
Embodiment 3
[0049] A method for extracting phycocyanin in cyanobacteria, comprising the steps of:
[0050] (1) Wall breaking: Mix blue-green algae and table salt at a mass ratio of 1:0.15, place in a vacuum freezer until the temperature is minus 15°C, grind to pass 60 mesh, then heat to a temperature of 30-35°C, and then add blue-green algae 1.8 times the mass of the isothermal extract was soaked at a constant temperature for 18 minutes; the extract was composed of the following parts by mass: 6 parts of sodium citrate, 15 parts of sodium lauryl sulfate, 4 parts of sodium tartrate, 11 parts of sodium dihydrogen phosphate, 85 parts parts deionized water;
[0051] (2) Coarse filtration: After filtering the broken wall product with a 0.8 μm microfiltration membrane, add oil and deionized water in turn to mix, and remove the oil through water and oil separation, and then filter with a 0.8 μm microfiltration membrane; The dosage is 18% of the mass of cyanobacteria, and the mixing time is 8min...
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