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Method for extracting phycocyanin from cyanobacteria

A phycocyanin and extraction method technology, applied in the field of protein extraction, can solve the problems of difficult industrialization, unsatisfactory yield, purity, and cost, and achieve the effects of short extraction cycle, low price and good stability

Active Publication Date: 2019-09-24
北海生巴达生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing technology is not ideal in terms of yield, purity and cost, so it is difficult to realize industrialization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for extracting phycocyanin in cyanobacteria, comprising the steps of:

[0034] (1) Wall breaking: mix blue-green algae and table salt at a mass ratio of 1:0.2, place in vacuum freezer until the temperature is minus 20°C, grind to 80 mesh, then heat to a temperature of 30-35°C, then add blue-green algae 2 times the mass of the isothermal extract was soaked at a constant temperature for 20 minutes; the extract was composed of the following parts by mass: 8 parts of sodium citrate, 17 parts of sodium lauryl sulfate, 6 parts of sodium tartrate, 12 parts of sodium dihydrogen phosphate, 90 parts parts deionized water;

[0035] (2) Coarse filtration: After filtering the broken wall product with a 0.5 μm microfiltration membrane, add oil and deionized water in turn to mix, and remove the oil through water and oil separation, and then filter with a 0.5 μm microfiltration membrane; The dosage is 20% of the mass of cyanobacteria, and the mixing time is 10min; the dosage ...

Embodiment 2

[0041] A method for extracting phycocyanin in cyanobacteria, comprising the steps of:

[0042] (1) Wall breaking: mix blue-green algae and table salt at a mass ratio of 1:0.1, put them in a vacuum freezer until the temperature is minus 10°C, grind to 50 meshes, then heat to a temperature of 30-35°C, and then add blue-green algae 1.5 times the mass of the isothermal extract is soaked at a constant temperature for 15-20 minutes; the extract is composed of the following parts by mass: 5 parts of sodium citrate, 13 parts of sodium lauryl sulfate, 2 parts of sodium tartrate, and 10 parts of sodium dihydrogen phosphate , 80 parts of deionized water;

[0043] (2) Coarse filtration: After filtering the broken wall product with a 1 μm microfiltration membrane, add oil and deionized water in turn to mix, and remove the oil through water and oil separation, and then filter with a 1 μm microfiltration membrane; the amount of oil The mass of cyanobacteria is 15%, and the mixing time is 5 ...

Embodiment 3

[0049] A method for extracting phycocyanin in cyanobacteria, comprising the steps of:

[0050] (1) Wall breaking: Mix blue-green algae and table salt at a mass ratio of 1:0.15, place in a vacuum freezer until the temperature is minus 15°C, grind to pass 60 mesh, then heat to a temperature of 30-35°C, and then add blue-green algae 1.8 times the mass of the isothermal extract was soaked at a constant temperature for 18 minutes; the extract was composed of the following parts by mass: 6 parts of sodium citrate, 15 parts of sodium lauryl sulfate, 4 parts of sodium tartrate, 11 parts of sodium dihydrogen phosphate, 85 parts parts deionized water;

[0051] (2) Coarse filtration: After filtering the broken wall product with a 0.8 μm microfiltration membrane, add oil and deionized water in turn to mix, and remove the oil through water and oil separation, and then filter with a 0.8 μm microfiltration membrane; The dosage is 18% of the mass of cyanobacteria, and the mixing time is 8min...

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Abstract

The invention relates to the technical field of protein extraction, in particular to a method for extracting phycocyanin from cyanobacteria. The method for extracting the phycocyanin from the cyanobacteria comprises the steps that cell disruption, coarse filtration and purification are carried out; and according to the coarse filtration, after products obtained from cell disruption are filtered by a microfiltration membrane of 0.5-1[mu]m, grease and deionized water are sequentially added for mixing, the grease is removed through water-oil separation, and then filtration is carried out by the microfiltration membrane of 0.5-1[mu]m. The method for extracting the phycocyanin from the cyanobacteria has the characteristics of high yield, high purity, high protein stability, short extraction cycle, low cost, simple operation and good resource utilization rate.

Description

technical field [0001] The invention relates to the technical field of protein extraction; in particular, it relates to a method for extracting phycocyanin from cyanobacteria. Background technique [0002] Effective substances contained in blue-green algae include amino acids, polysaccharides, phycobiliproteins, and algal toxins; It is widely used as a fluorescent reagent in natural pigments, medical and health products, and molecular new biology research. Phycocyanin is mostly extracted from spirulina, eyebrow algae, mat algae, etc., and there are few studies on the extraction of cyanobacteria phycocyanin. [0003] At present, phycocyanin is mainly extracted by purification methods such as extraction, coarse filtration, microfiltration, ultrafiltration, and low-temperature static centrifugation; such as the extraction and purification of phycocyanin by an activated carbon pretreatment combined with salting-out method disclosed in Patent No. CN201510692709.7 A method compr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/795C07K1/34C07K1/14C12N1/06
CPCC07K14/795C12N1/06
Inventor 薛命雄张玮瑲李玉芬梁振秀陈国珍
Owner 北海生巴达生物科技有限公司
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