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Microwave-assisted method for killing aspergillus flavus

A microwave-assisted, Aspergillus flavus technology, applied in the field of killing Aspergillus flavus, can solve the problems of difficult separation of enzymes and slow degradation speed, and achieve the effects of accelerated degradation, low cost and simple process

Pending Publication Date: 2019-09-27
GUANGXI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of degrading aflatoxin, additional substances such as enzymes are difficult to separate from the raw materials, and the degradation rate is slow

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Collect peanuts, corn, barley, soybeans, and wheat that are prone to the growth of Aspergillus flavus, and place them in a micro-electrolytic cell;

[0033] (2) Inject a sodium chloride solution with a mass fraction of 2% into the micro-electrolysis cell, submerge peanuts, corn, barley, soybeans, and wheat, and then start micro-electrolysis. The time of micro-electrolysis is 6 minutes, and the intensity is 1.2V / cm. The power is 600W, when the micro-electrolysis is started, the micro-electrolysis tank is aerated, and the aeration volume is controlled to be 35L / m 3 Min, the dissolved oxygen in the electrolyte is 0.8mg / L;

[0034] (3) After the micro-electrolysis is over, take peanuts, corn, barley, soybeans, and wheat out of the micro-electrolysis cell and place them in a microwave chamber; then pass through the ozone 6g / m 3 Start the microwave immediately after that, the microwave power is 1200W, the microwave frequency is 915MHz, the temperature is set to 40°C, the...

Embodiment 2

[0038] (1) Collect sugarcane that is prone to grow Aspergillus flavus and place it in a micro-electrolytic cell;

[0039] (2) Inject a sodium chloride solution with a mass fraction of 5% into the micro-electrolysis cell. After submerging the sugarcane, start the micro-electrolysis. The time of the micro-electrolysis is 10 minutes, the intensity is 2V / cm, and the power is 800W. Aeration is carried out in the micro-electrolytic cell, and the aeration volume is controlled at 50L / m 3 Min, the dissolved oxygen in the electrolyte is 1mg / L;

[0040] (3) After the micro-electrolysis is over, take the sugarcane out of the micro-electrolysis cell and place it in a microwave chamber; then pass through the ozone 10g / m 3 Start the microwave immediately afterward, the microwave power is 1000W, the microwave frequency is 433MHz, and the temperature is set to 45°C. The microwave is processed intermittently, working for 30s, then stopping for 20s, and repeating 5 times;

[0041] (4) After mi...

Embodiment 3

[0044] (1) Collect rice that is prone to grow Aspergillus flavus and place it in a micro-electrolytic cell;

[0045] (2) Inject a sodium chloride solution with a mass fraction of 1% into the micro-electrolysis cell. After submerging the rice, start the micro-electrolysis. The time of the micro-electrolysis is 5 minutes, the intensity is 0.5V / cm, and the power is 300W. Aeration is carried out in the micro-electrolytic cell, and the aeration volume is controlled to be 10L / m 3 Min, the dissolved oxygen in the electrolyte is 0.5mg / L;

[0046] (3) After the micro-electrolysis is over, take the rice out of the micro-electrolysis cell and place it in the microwave chamber; then pass through the ozone 5g / m 3 Immediately start the microwave, the microwave power is 500W, the microwave frequency is 915MHz, the temperature is set to 35°C, the microwave is processed intermittently, works for 20s, then pauses for 15s, repeats 6 times;

[0047] (4) After the microwave killing is completed,...

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Abstract

The invention discloses a microwave-assisted method for killing aspergillus flavus. Solid substances easy to grow the aspergillus flavus are subjected to microwave, micro-electrolysis and ozone combined treatment; the micro electrolysis time is 5-30 min; the ozone inlet amount is 5-10 g / m<3>; the microwave power is 500-1200 W, the microwave frequency is 300 MHz-2450 MHz, the temperature is set to be 35-45 DEG C, intermittent microwave treatment is implemented, the working time is 5-60 s, the interval time is 15-60 s, and microwave operation is repeated for 5-10 times. The method provided by the invention can kill the aspergillus flavus and spores thereof in crops such as corn, wheat, rice and sugarcane as well as feeds, and the killing rate of the aspergillus flavus is 90-97%, so that the capability of producing toxin by the aspergillus flavus is reduced, no chemical residue is generated, no pollution is caused to the environment, the product safety is high, and the method has a good development prospect in the fields of food, feed processing and the like.

Description

technical field [0001] The invention belongs to the field of killing aspergillus flavus, and in particular relates to a microwave-assisted method for killing aspergillus flavus. Background technique [0002] Aspergillus flavus, half known fungi, a common saprophytic fungus. It is more common in moldy grains, grain products and other moldy organic matter, and aflatoxins are easily produced after polluting food crops. Aflatoxin is the most important mycotoxin that pollutes grain and oil crops, food and feed in my country and the world. It is highly toxic and carcinogenic, and seriously endangers the health of humans and animals. In 1993, aflatoxin B1 was listed as a class I carcinogen by the World Health Organization, and it is one of the strongest known carcinogens. [0003] Chinese patent CN01107435.3 discloses a detoxification method of aflatoxin in brewing condiment raw materials, which is characterized in that Pseudomonas or Aspergillus niger are fermented and mechanica...

Claims

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Application Information

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IPC IPC(8): A23L5/20A23L5/30
CPCA23L5/34A23L5/20A23L5/27Y02P60/85
Inventor 谢能中黄志民李秉正陈先锐黄纪民
Owner GUANGXI ACAD OF SCI
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