Phospholipid nano-disk chromatography medium and preparation method and application thereof

A technology of phospholipid nanodiscs and chromatographic media, applied in the biological field, can solve the problems of less information, tedious and lengthy operation process, loss of membrane protein activity, etc.
CN110292916AActive Publication Date: 2019-10-01INST OF PROCESS ENG CHINESE ACAD OF SCI +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF PROCESS ENG CHINESE ACAD OF SCI
Publication Date
2019-10-01

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Abstract

The invention relates to a phospholipid nano-disk chromatography medium and a preparation method and application thereof. The phospholipid nano-disk chromatography medium comprises a charged phospholipid nano disk and an affinity matrix; the charged phospholipid nano disk comprises phospholipid and a film scaffold protein with an affinity label; the charged phospholipid nano disk is connected withan affinity matrix through the affinity label. The phospholipid nano-disk chromatography medium has both binding capacity and protection capacity to film proteins, so that the actual system containing the film proteins can be purified and reconstructed on a chromatography column in one step; and the phospholipid nano-disk chromatography medium has reversibility and regeneration performance; the phospholipid nano-disk chromatography medium solves the problems of serious protein activity loss, long and complicated steps and time-consuming and labor-consuming operation in the traditional film protein research technology, is simple and efficient to operate, improves the film protein activity recovery rate by more than 20 times, and shortens the treatment time by more than 95%.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a chromatographic medium for separating and purifying membrane proteins, in particular to a phospholipid nanodisc chromatographic medium and its preparation method and application. Background technique

[0002] Membrane proteins, as the main bearers of biomembrane functions, not only provide a stable internal environment for the life activities of individual cells, but also mediate the connection between cells and cells, and between cells and substrates, thus playing an important role in the signal transduction and physiological transport of living organisms. , Enzyme catalysis and structural connection play a rich and important function. Therefore, membrane proteins have always been the focus of research in the fields of biology, medicine and materials. However, membrane proteins are also recognized as research difficulties. The vast majority of membrane proteins are e...

Claims

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