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Method for measuring concentration of carboxyl polystyrene microspheres by using functionalized capillary

A technology of polystyrene microspheres and capillaries, which is applied in the field of optical sensing, can solve the problems of less detection-related technologies, and achieve low cost, no photobleaching, and good repeatability

Active Publication Date: 2019-10-08
UNIV OF ELECTRONICS SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the capillary in laser mode has few related technologies for the detection of specific molecules.

Method used

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  • Method for measuring concentration of carboxyl polystyrene microspheres by using functionalized capillary
  • Method for measuring concentration of carboxyl polystyrene microspheres by using functionalized capillary
  • Method for measuring concentration of carboxyl polystyrene microspheres by using functionalized capillary

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Embodiment

[0041] A method for utilizing a functionalized capillary to measure the concentration of carboxypolystyrene microspheres provided by a preferred embodiment of the present invention, the specific steps are as follows:

[0042] S1. Put capillary tubes with inner and outer diameters of 125 μm and 160 μm and a length of 5 cm in a 923K furnace with oxygen to remove the outer polyimide polymer film to obtain a processed capillary, and both ends are connected to a pump for liquid On the plastic tube, put it on the microscope stage;

[0043] S2. Use 10mol / L NaOH solution to pump 0.1mL / min for 2 minutes to the treatment capillary obtained in S1 step, so that the glass inner surface of the capillary is slightly negatively charged; then use 2mg / mL PAH solution and 2.5mol / L The mixed solution of NaCl solution is pumped at 0.02mL / min for 5 minutes, and the slightly positive charge on the surface of PAH can attract the negative charge on the surface of the glass above to form a PAH-PSS film...

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Abstract

The invention discloses a method for measuring concentration of carboxyl polystyrene microspheres by using a functionalized capillary, and the method firstly forms an optical geometric resonance modenear the glass wall by pumping rhodamine-B in a thick-walled capillary cavity, prepares a high-molecular polymer film on the inner wall of the capillary to bond particles of a specific functional bond, and finally uses discrete Fourier transform to analyze the displacement of geometry modes including a triangular mode and a star mode. The method provided by the invention performs directed measurement on the particles of the specific functional bond, and has the advantages of high sensitivity, low cost, simple measurement method, stability, good repeatability, higher intensity of the resonancelaser mode than the whispering wall mode, low detection limit and no photo-bleaching.

Description

technical field [0001] The invention belongs to the field of optical sensing, in particular to a method for measuring the concentration of carboxyl polystyrene microspheres by using a functionalized capillary. Background technique [0002] Optical resonators based on whispering gallery modes, including microspheres, microrings, and capillaries, are widely used in sensing. Whispering gallery mode-based sensing is based on two methods of measuring the mode structure. One is to use waveguides, prisms or other special devices to couple evanescent waves to near-resonant cavities. When a particle or a biomolecule to be detected attaches to the chamber wall, it causes a shift in the resonance mode. Evanescent field coupling needs to consider many factors such as phase matching. Resonant modes of this method have high quality factors of 109 or higher and can be used for single-molecule detection. However, this method also has many shortcomings that limit its development, such as...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/06
CPCG01N15/06G01N15/075
Inventor 张志红杨赛龙尚思晖
Owner UNIV OF ELECTRONICS SCI & TECH OF CHINA
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