Supercharge Your Innovation With Domain-Expert AI Agents!

Colorimetric detection method for kanamycin residue in milk

A kanamycin and detection method technology, applied in the field of analytical chemistry, can solve the problems of expensive reagents, long detection time, complex instruments, etc., and achieve the effects of convenient operation, low cost and low sensitivity

Inactive Publication Date: 2019-10-11
NANJING AGRICULTURAL UNIVERSITY
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of these methods have certain defects, cumbersome sample pretreatment procedures, expensive reagents for immunochemical methods, long detection time, complicated instruments

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Colorimetric detection method for kanamycin residue in milk
  • Colorimetric detection method for kanamycin residue in milk
  • Colorimetric detection method for kanamycin residue in milk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1. Determination of the specificity of a colorimetric detection method for kanamycin residues in milk

[0030] Under the optimal experimental conditions, the system specificity verification was carried out. The CP prepared in the above method step (2) KNA / APT KNA Modified SDB 4μL, 4μL of different antibiotics and 2μL of 10mM PBS (pH 7.4) containing 0.1M NaCl were prepared as a mixed solution, incubated at 37°C for 1h, washed 3 times with 10mM PBS (pH 7.0), and then added 12.5μL of the above steps (4 ) in SP KNA / HP KNA The modified AuNPs were incubated at 37°C for 1h, washed and resuspended with 10mM PBS (pH 7.0), added 0.5μL of SAv-HRP (0.01mg / mL) and incubated at 37°C for 1h, washed 5 times with 200μL of 10mM PBS (pH 6.0) , add 600 μL OPD-H 2 o 2The resuspended magnetic beads were incubated at 37°C for 30 min to observe the color change and detect the UV absorbance at 450 nm. The measured scan results are as figure 2 A, As shown in 2B, the method ...

Embodiment 2

[0031] Embodiment 2. In the presence of different concentrations of kanamycin, the determination of the concentration standard curve of UV absorbance and kanamycin

[0032] Prepare a mixture of 4 μL of SDB prepared in step (2) of the above method, 4 μL of different concentrations of kanamycin and 2 μL of 10 mM PBS containing 0.1M NaCl and a pH value of 7.4, and incubate at 37°C for 1 hour, 10 mM PBS (pH 7.0) After washing, add 12.5 μL of SP in the above step (4) KNA / HP KNA The modified AuNPs were incubated at 37°C for 1h, washed with 10mM PBS (pH 7.0) and resuspended, then added 0.5μL of SAv-HRP (0.01mg / mL) and incubated at 37°C for 1h, then washed with 200μL of 10mM PBS (pH 6.0) , add 600 μL OPD-H 2 o 2 (contains 7.4mM OPD and 5.9mM H 2 o 2 20mM phosphate-citrate buffer, pH 5.0) to resuspend the magnetic beads and incubate at 37°C for 30min to observe the color change and detect the UV absorbance at 450nm. The test results are as image 3 As shown in A-C, when the con...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A colorimetric detection method for kanamycin residue in milk belongs to the technical field of analysis chemistry. The colorimetric detection method comprises the steps of performing annealing hybridization on a kanamycin aptamer (APTKNA) and a biotin-marked probe CPKN to form dual-chain DNA modified on a surface of a streptavidin coupling magnetic bead (SDB), and modifying probes SPKNA and HPKNAon a surface of gold nanoparticle (AuNPs); and allowing KNA and the APTKNA to be specially combined and allowing the APTKNA to be separated from the SDB when kanamycin (KNA) exists in a system, and performing hybridization on the CPKNA and AuNPs-modified HPKNA to form an SDB-AuNPs system. An OPD-H2O2 solution can be catalyzed to change color after AuNPs surface-modified SPKNA is combined with SAv-HRP, a standard curve is drawn according to a relation between change of ultraviolet light absorption degree at 450 nanometers and kanamycin concentration, and the detection of the kanqamycin concentration can be achieved. The method has the characteristics of high sensitivity, low cost and the like and is easy to operate, and sensitive measurement of the kanamycin in a sample can be achieved.

Description

technical field [0001] The invention relates to a colorimetric detection method for kanamycin residues in milk, in particular to a detection method for kanamycin residues in milk, belonging to the field of analytical chemistry. Background technique [0002] Kanamycin is an effective aminoglycoside broad-spectrum antibiotic for the treatment of Gram-negative and Gram-positive bacterial infections. It is one of the most commonly used anti-infective drugs in clinical practice. And long-lasting, it is widely used in human and animal injection, oral and other forms of treatment. However, excessive use of kanamycin or long-term consumption of animal-derived foods containing kanamycin residues can lead to a series of adverse reactions, including ear and kidney toxicity and anaphylactic shock. Therefore, the establishment of a detection method for food-derived antibiotic residues is of great significance to actual production and life. [0003] Gold nanoparticles are widely used as...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/33G01N33/531G01N33/543
CPCG01N21/33G01N21/78G01N33/531G01N33/54326G01N33/54346
Inventor 许媛媛苗晋锋张源淑
Owner NANJING AGRICULTURAL UNIVERSITY
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com