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Soil infiltration system metabonomics analysis method based on UPLC-MS

A technology of metabolomics and analysis methods, applied in the direction of analysis materials, scientific instruments, material separation, etc., can solve the problem of inability to accurately reflect the complete biological information of SIS, the health reliability of SIS is not strong, and the quality of effluent water and biological flora cannot be immediately resolved. Make adjustments and other issues to achieve the effect of rigorous technical solutions, reasonable and reliable model design, and simple and easy analysis methods

Inactive Publication Date: 2019-10-11
NORTHEASTERN UNIV
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Problems solved by technology

[0005] However, at present, the research on the ecological characteristics of microorganisms in SIS still stays in the traditional microbial analysis method. When using traditional molecular biology methods to study SIS, when the system is disturbed, the effluent water quality and biological flora cannot be adjusted immediately.
This simple traditional microbial analysis method cannot accurately reflect the complete biological information of the SIS, and it is not reliable to intuitively determine whether the SIS is healthy or not.

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  • Soil infiltration system metabonomics analysis method based on UPLC-MS
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Embodiment Construction

[0038] The following is a detailed description of the technical solution of the present invention in conjunction with the accompanying drawings.

[0039] Such as figure 1 As shown in the flowchart of the UPLC-MS-based metabolomics analysis method for soil infiltration system, the specific steps of a metabolomics analysis method for soil infiltration system based on UPLC-MS are as follows:

[0040] Step 1: Sample preparation: collect soil samples, and use liquid chromatography-electrospray time-of-flight mass spectrometry system for UPLC-MS detection and analysis after pretreatment. In this example, a total of 72 soils in the soil infiltration system (SIS) were collected. Samples were divided into 3 groups on average, group A (organic load 250mg L -1 ), Group B (with an organic load of 400mg·L -1 ), Group C (the organic load is 500mg·L -1 ), and then carry out pretreatment to above-mentioned A, B, C three groups of samples of collection, the reagent used during pre...

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Abstract

The invention discloses a soil infiltration system metabonomics analysis method based on UPLC-MS. The method comprises the following process steps of 1) carrying out sample preparation: collecting a soil sample, and performing UPLC-MS detection analysis by adopting a liquid chromatography-electrospray time-of-flight mass spectrometry system after pretreatment; 2) obtaining sample data through theUPLC-MS detection technology, and carrying out extraction, noise filtering, deconvolution, alignment and normalization on the sample data; 3) carrying out multivariate statistical analysis on the dataafter the normalization processing; 4) screening and identifying and analyzing the differential metabolites; 5) analyzing a microorganism metabolic pathway; and 6) carrying out redundant (RDA) analysis of metabolites and environmental factors. The soil infiltration system metabonomics analysis method based on UPLC-MS is simple and easy to implement, can comprehensively reflect the difference condition of the soil microorganism metabolites, is reasonable and reliable in model design, and provides a quick, efficient and accurate analysis method for researching soil system microorganism metabonomics.

Description

technical field [0001] The invention belongs to the field of biotechnology, relates to the research on microbial metabolism in a soil infiltration system, and provides a method for research on microbial metabolic mechanism. Background technique [0002] Soil infiltration system (SIS) is a sewage ecological treatment technology with low investment, low energy consumption and low operating cost. SIS contains a large number of various microorganisms. The action of microorganisms is an important way for SIS to decontaminate. The metabolic process and product characteristics of microorganisms are an important basis for qualitatively describing the pollutant removal process. [0003] Microbial metabolomics refers to the simultaneous qualitative and quantitative analysis of all low-molecular-weight metabolites existing inside and outside the cell in an unbiased and reproducible manner at a specific time point in microbial cell growth or growth cycle. , analysis of metabolic pathwa...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8606G01N30/8679G01N30/8696G01N2030/045
Inventor 李英华杨蕾李海波苏菲戴陆明杨晨
Owner NORTHEASTERN UNIV
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