Soil infiltration system metabonomics analysis method based on UPLC-MS
A technology of metabolomics and analysis methods, applied in the direction of analysis materials, scientific instruments, material separation, etc., can solve the problem of inability to accurately reflect the complete biological information of SIS, the health reliability of SIS is not strong, and the quality of effluent water and biological flora cannot be immediately resolved. Make adjustments and other issues to achieve the effect of rigorous technical solutions, reasonable and reliable model design, and simple and easy analysis methods
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[0038] The following is a detailed description of the technical solution of the present invention in conjunction with the accompanying drawings.
[0039] Such as figure 1 As shown in the flowchart of the UPLC-MS-based metabolomics analysis method for soil infiltration system, the specific steps of a metabolomics analysis method for soil infiltration system based on UPLC-MS are as follows:
[0040] Step 1: Sample preparation: collect soil samples, and use liquid chromatography-electrospray time-of-flight mass spectrometry system for UPLC-MS detection and analysis after pretreatment. In this example, a total of 72 soils in the soil infiltration system (SIS) were collected. Samples were divided into 3 groups on average, group A (organic load 250mg L -1 ), Group B (with an organic load of 400mg·L -1 ), Group C (the organic load is 500mg·L -1 ), and then carry out pretreatment to above-mentioned A, B, C three groups of samples of collection, the reagent used during pre...
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