A microfluidic device and screening method for tumor cell screening based on droplet registration

Abstract

The invention relates to a microfluidic device and a screening method for tumor cell screening based on droplet storage, belonging to the field of microfluidic technology. The present invention includes a glass substrate and a PDMS chip fixed thereon, wherein the PDMS material constitutes the entire chip, and a microchannel structure is made by microfabrication technology, a droplet is generated through a cross-shaped structure to wrap a red blood cell or a tumor cell, and then passed through the liquid Droplet register passive valve, due to the difference in size and softness between red blood cells and tumor cells, the number of droplets that wrap red blood cells and those that wrap tumor cells pass through the droplet register is different, and the number of droplets that pass through the droplet register is detected by laser , so as to detect whether there are tumor cells, and realize high-sensitivity real-time detection with a very small sample amount. The invention realizes the integration of fluid sampling, driving, manipulation, transportation and detection and analysis on the microfluidic chip, and realizes controllable liquid flow, transportation of micro and trace substances and efficient detection.

Description

technical field [0001] The invention relates to a microfluidic device and a screening method for tumor cell screening based on droplet storage, belonging to the field of microfluidic technology. Background technique [0002] Microfluidic chips are widely used in disease diagnosis, drug screening, environmental testing, food safety and other fields. One of the basic points of the microfluidic chip is to operate and control the fluid at the micro scale, and the amount of fluid as the object of operation and control is extremely small, and many manifestations of substances are different from constant substances. Tumor is one of the most dangerous diseases that threaten human life and health. The root cause of its occurrence is the abnormality of the mechanism of controlling cell growth and proliferation, which is related to the abnormality of cell apoptosis. The traditional medical detection methods for tumor cells have low detection efficiency. Sincerely. Therefore, how to q...

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Problems solved by technology

Tumor is one of the most dangerous diseases that threaten human life and health. The root cause of its occurrence is the abnormality of the mechanism of controlling cell growth and proliferation, which is related to the abnormality of cell apoptosis. The traditional medical detection methods for tumor cells have low detection efficiency. To

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