Separation and preparation method of pancratistatin
A semi-prepared technology of splanadin, which is applied in the field of medicine, can solve the problems of high cost, cumbersome steps, and low yield, and achieve the effects of shortened reflux time, high extraction rate, and high purity
Pending Publication Date: 2019-10-18
KUNMING MEDICAL UNIVERSITY
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[0005] At present, methods such as methanol, ethanol cold immersion and percolation are used for the extraction of splanadin in Scutellaria sativa, which are costly, use a large amount of organic solvents, easily cause pollution to the environment, and are time-consuming; preparation of multi-purpose dextran Gel LH-20, silica gel and other separation techniques, the steps are cumbersome and the yield is low
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[0029] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
[0030] refer to Figure 1-7 , a method for the separation and preparation of sanaline, characterized in that it comprises the following steps:
[0031] (a) Extraction: the plant Shuiguiban is collected, its rhizomes are washed and chopped, and methanol is used as a solvent with a liquid-to-material ratio of 3:1 for reflux extraction, and the liquid-to-material ratio is the volume-to-mass ratio;
[0032] (b) Extraction: use a rotary evaporator to remove methan...
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The invention belongs to the technical field of medicines, and relates to a separation and preparation method of pancratistatin. The separation and preparation method comprises the following steps: extracting is carried out, specifically, plant hymenocallis littoralis is picked, root stocks of the plant hymenocallis littoralis are taken to be washed and cut up, and methyl alcohol serves as a solvent for reflux extracting; abstracting is carried out, specifically, methyl alcohol in a methyl alcohol extracting solution is removed through a rotary evaporation instrument, only an aqueous phase isretained finally, the aqueous phase is repeatedly abstracted for ten times through ethyl acetate, and after an ethyl acetate abstracting solution is evaporated completely, an extract sample is obtained; elution and collecting are carried out, specifically, after an ethyl acetate extract obtained in the abstracting step is extracted, the methyl alcohol is selected to dissolve the extract, and the extract is eluted and collected through a dichloromethane and methyl alcohol mixed solution; and half-preparation high performance liquid chromatography (HPLC) separation is carried out, specifically,collecting fluid eluted and collected through the dichloromethane and methyl alcohol mixed solution in elution and collecting is evaporated completely through rotary evaporation, and the high-purity pancratistatin is prepared through half-preparation HPLC separation. The preparation method is high in preparation speed, and the purity of a prepared finished product is high.
Description
technical field [0001] The invention belongs to the technical field of medicines, and relates to a method for separating and preparing schianaline. Background technique [0002] Hymenocallis littoralis, also known as spider orchid, is a plant of the genus Hymenocallis littoralis in the family Amaryllidaceae. It has been used as folk medicine since ancient times. According to the records in "Chinese Materia Medica", it is warm in nature and pungent in taste. Its leaves and bulbs are used as medicine to have the effects of anti-tumor, anti-virus, relaxing muscles and blood circulation, reducing swelling and relieving pain. The chemical composition of Scutellaria is complex, including alkaloids, flavonoids and phenols, among which alkaloids are its main active ingredients. The known alkaloids currently include narciscycline, lycorine, sanaline, 7-de Oxynarcicycline, 7-deoxy-trans-dihydronarcicycline, polyflora narcisine, Zhangzhou narcisine, homolycorine, lycorine, O-methyl ly...
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Login to View More IPC IPC(8): C07D491/056
CPCC07D491/056
Inventor 刘丹丹李霁梁蕾蕾胡建林吴双凤刘曼曼
Owner KUNMING MEDICAL UNIVERSITY