Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Immunomagnetic beads and preparation method thereof

An immunomagnetic bead, uniform technology, applied in the preparation of microspheres, inductance/transformer/magnet manufacturing, magnetic materials, etc., can solve the problems of poor magnetic responsiveness, high price, uneven particle size, etc.

Active Publication Date: 2019-10-18
DONGGUAN DONGYANG SOLAR SCI RES & DEV CO LTD
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Foreign commercial magnetic beads are expensive, and the technology is strictly confidential. Domestic magnetic beads generally have defects such as uneven particle size, poor magnetic response, and inconsistent magnetic response. What is more serious is that nano-magnetic particles and microspheres appear during use. Shedding of contaminated samples

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Immunomagnetic beads and preparation method thereof
  • Immunomagnetic beads and preparation method thereof
  • Immunomagnetic beads and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] 1. Swelling of polystyrene microspheres

[0071] Take 2g of polystyrene microspheres (1μm) and disperse them into 100mL of 0.1% SDS aqueous solution, then add 20mL of absolute ethanol and 5g of dibutyl phthalate, fully ultrasonically emulsify and add to a four-necked flask with a mechanical stirrer , stirred at a water bath temperature of 50° C. for 10 h, centrifuged to separate white solid particles after swelling, washed with pure water and absolute ethanol for 3 to 5 times, and then used for later use.

[0072] 2. Synthesis of magnetic composite microspheres

[0073] Dissolve 1.25g NaOH in 50mL EG, then add the swollen polystyrene microspheres into it, stir vigorously to disperse them, and then perform ultrasonic to form a uniform emulsion to obtain emulsion A; take another 10mL EG, 5gFeCl 3 ·6H 2 O and 10mLH 2 O mixed with ultrasound to obtain solution B. Slowly add solution B to emulsion A while stirring vigorously. After the dropwise addition, continue to stir ...

Embodiment 2

[0084] Adjust the water consumption of solution B in the second step of Example 1 to 18mL, adjust the pressure to 0.6MPa, raise the temperature to 210°C, extend the reaction time to 20h, and keep other process steps, parameters and operations unchanged. Finally, the performance of the magnetic beads was obtained in the experiment. And structure is close to embodiment 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention provides immunomagnetic beads and a preparation method thereof. The preparation method comprises the following steps: swelling polystyrene microspheres; synthesizing magnetic composite microspheres at high temperature and high pressure; carrying out acid dissolution to remove exposed magnetic particles on the surfaces of the magnetic composite microspheres; and carrying out surface cross-linking modification on the magnetic composite microspheres. The immunomagnetic beads prepared by the method disclosed by the invention are uniform in particle size, good in magnetic responsiveness, stable in structure and consistent in magnetic content.

Description

technical field [0001] The invention relates to the technical field of composite material preparation, in particular to a preparation method of immunomagnetic beads. Background technique [0002] Magnetic beads are composite functional microspheres with superparamagnetism. Because of their surface modification with functional groups such as amino groups and carboxyl groups, they can be coupled to proteins, DNA, etc. Therefore, it has a wide range of applications in cell separation, immobilized enzymes, immunodiagnosis, tumor targeted therapy, and DNA extraction. [0003] Chemiluminescent immunomagnetic beads have higher requirements among all magnetic beads, and require suitable particle size, high particle size uniformity, magnetic response consistency, etc. for magnetic microspheres. Foreign commercial magnetic beads are expensive, and the technology is strictly confidential. Domestic magnetic beads generally have defects such as uneven particle size, poor magnetic respon...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08F257/02C08F212/36C08F220/06C08K3/22B01J13/14H01F1/00H01F41/00
CPCC08F257/02C08K3/22B01J13/14H01F1/0018H01F41/00C08K2003/2275C08K2201/011C08F212/36C08F220/06
Inventor 王鹏侯立威吴志能谌庆春李向东张军
Owner DONGGUAN DONGYANG SOLAR SCI RES & DEV CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com