Preparation and application of recombinant protein cfp-10 nanoparticles

A technology of CFP-10 and recombinant protein, applied in medical preparations of non-active ingredients, antibacterial drugs, microcapsules, etc., can solve the problems of missing RD1 region and limited protective effect of adults, etc., to improve IgA level and promote BCG immune effect, effect of reducing tissue load
CN110354098BActive Publication Date: 2021-01-05CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2021-01-05

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Abstract

The invention provides a preparation method of recombinant protein CFP-10 nanoparticles and the application thereof in preparing tuberculosis prevention vaccines. The preparation method includes the steps of (1) configuration of a polylactic acid-glycolic acid copolymer PLGA solution; (2) configuration of a recombinant protein CFP-10 solution; (3) preparation of primary emulsion; (4) preparation of compound emulsion; (5) formation of the nanoparticles. The recombinant protein CFP-10 nanoparticles prepared by the preparation method are used for enhancing the immunity of BCG-immunized mice through intranasal drip, and it is confirmed that the recombinant protein CFP 10 nanoparticles have the effects of improving mucosal immunity level and promoting BCG immunity.
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Description

technical field

[0001] The invention belongs to the field of new vaccine applications, and in particular relates to the preparation of recombinant protein CFP-10 nanoparticles and its application in the preparation of tuberculosis prevention vaccines. Background technique

[0002] Bovine tuberculosis is an important zoonotic disease, which endangers the development of breeding industry and public health security. The causative bacterium of bovine tuberculosis is Mycobacterium bovis, which can break through the interspecies barrier and spread between other mammals and humans. It has a wide range of hosts and affects many mammal groups. The current investigation and research shows that bovine tuberculosis in my country has the characteristics of easy recurrence and national distribution, and it is transmitted to humans through food and contact. Bacillus Calmette-Guerin (BCG) is the most widely used vaccine to prevent tuberculosis. It has a significant protective effect on chi...

Claims

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