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Lactobacillus plantarum and application thereof

A technology of Lactobacillus plantarum and strains, applied in the field of microorganisms, can solve the problems of large difference in antioxidant capacity, difference in antioxidant capacity, etc.

Pending Publication Date: 2019-10-25
GUANGDONG YIKEWEI BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many types of Lactobacillus and Bifidobacteria that have been reported to have antioxidant functions. In different research reports, the antioxidant capacity of the same species of Lactobacillus varies greatly, and even different strains of the same species have higher antioxidant capacity in different studies. big difference

Method used

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  • Lactobacillus plantarum and application thereof
  • Lactobacillus plantarum and application thereof
  • Lactobacillus plantarum and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0032] 2. Preparation of experimental materials:

[0033] (1) Preparation of live Lactobacillus plantarum bacteria suspension: Lactobacillus plantarum was prepared according to 10 7 The inoculum amount of CFU / ml was inoculated in 500ml of MRS liquid medium, cultured anaerobically at 37°C for 18h, centrifuged at 4500r / min for 15min to collect the bacteria, and the bacteria pellet was resuspended in 10% (w / v) degreased Milk, respectively prepared into 2 × 10 6 The low-dose bacterium suspension L1 (embodiment 1) of CFU / ml, 2 * 10 8 The middle dosage bacterial suspension M1 (embodiment 2) of CFU / ml, 2 * 10 10 High-dose bacterial suspension H1 of CFU / ml (Example 3).

[0034] (2) Preparation of dead Lactobacillus plantarum suspension: Lactobacillus plantarum was prepared according to 10 7 The inoculation amount of CFU / ml was inoculated in 100ml of MRS liquid medium, cultured at 37°C for 18 hours, inactivated at 70°C for 1 hour, collected by centrifugation at 4500r / min for 15 min...

Embodiment 1-5

[0049] As can be seen from Table 2, compared with Comparative Example 3 (6.94g in body weight), the weight gain of mice in Comparative Example 2 (4.78g in body weight) slowed down, indicating that modeling had an adverse effect on the growth of mice. The weight gain of the mice in Examples 1-5 is higher than that in Comparative Example 2, indicating that Lactobacillus plantarum FEED8 can promote the weight gain of mice. Among them, in Examples 1-3, as the concentration increases, the body weight of mice increases by 5.4%-40.8% compared with Comparative Example 2, indicating that the concentration of Lactobacillus plantarum FEED8 is positively correlated with the body weight of mice. Compared with ratio 1, the difference in body weight of the mice in Examples 1-5 also has an impact, but the impact is not significant.

[0050] Table 3 Effect of Lactobacillus plantarum FEED8 on mouse serum GSH-Px activity, SOD activity, T-AOC content, and MDA content

[0051]

[0052] As can ...

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Abstract

The invention relates to the technical field of microorganisms, in particular to a lactobacillus plantarum FEED 8 and an application thereof. The lactobacillus plantarum has the preservation number ofCGMCC No. 15029, and is separated from the intestinal tract of a long-lived old man in Bama, Guangxi. Through mouse lavage, compared with a blank control group, the SOD activity, GSH-Px activity andT-AOC content in serum, brain and liver of a rat in the lactobacillus plantarum group are significantly increased, the MDA content is significantly decreased, and the degree of increase and decrease is positively correlated with the bacteria concentration, wherein, at the same concentration, the antioxidant effect of living bacteria is better than that of the mixture of living bacteria and dead bacteria, and better than that of dead bacteria. The lactobacillus plantarum is very suitable for the preparation of food, health care products, drugs, food supplements and other products.

Description

technical field [0001] The invention relates to the technical field of microorganisms, and more specifically relates to plantaractobacillus and its anti-oxidation application. Background technique [0002] Aging is a universal law of organisms in nature. Regarding the mechanism of aging, the most widely recognized theory is the free radical theory proposed by Harman et al. in 1957. This theory holds that the body will produce by-products of reactive oxygen species (reactive oxygen species, ROS) during normal oxidation, leading to the accumulation of endogenous free radicals, which will damage the cells of the body, resulting in intracellular lipids, proteins, DNA, etc. damage. [0003] Under normal circumstances, intracellular ROS levels and antioxidant protection systems are in a dynamic balance. When the body is in a certain state, such as infection, the production of ROS will increase. Exogenous factors such as ultraviolet radiation, ionizing radiation, chemotherapy, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P39/06A23L33/135C12R1/25
CPCA61K35/747A61P39/06A23L33/135A23V2002/00C12R2001/25C12N1/205A23V2400/169A23V2200/302
Inventor 余保宁闫刘慧金庭飞王然
Owner GUANGDONG YIKEWEI BIOLOGICAL TECH CO LTD
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