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Application of cotton GhMADS41-A04 gene in plant flowering promotion

A ghmads41-a04, gene technology, applied in the field of plant genetic engineering, can solve the problem of insufficient early maturity of short-season cotton, and achieve the effect of early flowering time

Inactive Publication Date: 2019-10-25
INST OF COTTON RES CHINESE ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current short-season cotton has the problem of insufficient early maturity

Method used

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  • Application of cotton GhMADS41-A04 gene in plant flowering promotion
  • Application of cotton GhMADS41-A04 gene in plant flowering promotion
  • Application of cotton GhMADS41-A04 gene in plant flowering promotion

Examples

Experimental program
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Effect test

Embodiment 1

[0027] 1. Cotton material

[0028] The cotton materials selected in this example are Upland cotton 50, Zhongmian Institute 74, Guoxin cotton No. 11, and Bomian No. 1, which were planted in the State Key Laboratory of Cotton Biology, Cotton Research Institute, Chinese Academy of Agricultural Sciences (Anyang Baibi) , the management measures are normal field management.

[0029] 2. Reagents and consumables

[0030] 2.1 Enzymes and kits: GXL DNA Polymerase High-Fidelity Enzyme, Gel Recovery Kit, and PCR Product Purification Kit were purchased from Takara Company; RNA Reverse Transcription Kit, KOD FX Neo Enzyme (Code.No.KFX-201) were purchased from Toyobo Company; The Ultra One Step Cloning Kit kit was purchased from Vazyme Company; a small amount of plasmid extraction kit was purchased from Magen Company; restriction endonucleases (BamH I, Sac I) were purchased from NEB Company; DNAMarker III and plant total RNA extraction kits were purchased from TIANGEN Company; fluoresce...

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Abstract

The invention discloses application of a cotton GhMADS41-A04 gene in plant flowering promotion and belongs to the technical field of plant gene engineering. The GhMADS41-A04 gene has a nucleotide sequence shown as SEQ ID NO:1 and an encodable amino acid sequence shown as SEQ ID NO:2. The flowering time of an arabidopsis thaliana plant with the transgenic GhMADS41-A04 gene obtained by a transgenictechnology is remarkably advanced. Furthermore, by silencing the GhMADS41-A04 gene in cotton, results suggest that the GhMADS41-A04 gene plays a key role in cotton flowering promotion. Favorable generesources are provided for short-season cotton cultivation.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and in particular relates to the application of GhMADS41-A04 gene in promoting flowering of plants. Background technique [0002] Cotton is one of the most important economic crops in our country and plays an important role in national production. However, the development of cotton is limited to a certain extent due to the land competition between grain and cotton. Breeding and promotion of short-season cotton varieties can alleviate the conflict between grain and cotton land, and is an effective way to achieve double harvests of grain and cotton. However, the current short-season cotton has the problem of insufficient prematurity. [0003] As one of the important traits of cotton, early maturity has become an important breeding goal of upland cotton. Studies have shown that flowering sooner or later is closely related to the early maturity of cotton. Therefore, flowering-rel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/00A01H6/46A01H6/20A01H6/60
CPCC07K14/415C12N15/827
Inventor 喻树迅程帅帅马亮王寒涛魏恒玲芦建华李林
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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