Method for increasing propagation force of milters through specific high expression gene in gonadal tissue

Abstract

The invention belongs to the technical field of gene expression, and discloses a method for increasing propagation force of milters through specific high expression gene in gonadal tissue. The methodcomprises the following steps of extracting RNA of the gonadal tissue, performing reverse transcription to obtain cDNA, using the cDNA as a formwork, using tesk1-F / tesk1-R as a primer, and performingPCR amplification on high-fidelity enzyme pfu, wherein the used carrier is a plasmid vector containing a kop promoter and nanos 3'UTR, the kop promoter and the nanos3'UTR can specifically express a target gene in reproduction cells, through NcoI and BseAI enzyme cutting, tesk1 gene is inserted between the kop promoter and the nanos3'UTR, and a genetic modification carrier is formed; and performingmicroinjection to obtain genetically engineered milters. In the gonadal tissues of the milters, the tesk1 gene is subjected to specific high expression, the inventor finds that the sexual gland indexes of the milters can be notably increased, sperm generation amount is increased, and the purpose of improving reproductive capacity of male zebra fish can be realized.

Description

technical field [0001] The invention belongs to the technical field of gene expression, and in particular relates to a method for increasing the fecundity of male fish with specific highly expressed genes in gonad tissue. Background technique [0002] At present, the closest existing technology: the reproductive capacity of a fish directly determines the degree of its application and promotion. One of the main reasons why the large-scale commercial farming of the four major species of carp can be promoted nationwide is that large-scale artificial breeding can be achieved and a large number of seedlings can be obtained. However, for some famous and high-quality fish such as eel and grouper, the biggest bottleneck in the promotion of breeding is the poor reproductive ability and the inability to obtain a large number of seedlings, which also keeps the prices of these fish high all the year round. Therefore, improving the fecundity of fish has very important significance for t...

AI Technical Summary

Problems solved by technology

[0007] (1) At present, the ratio of male to female of some fish is seriously out of balance, and there are very few male fish; the lack of male individuals severely restricts their reproductive scale, which prevents their further promotion and breeding

[0008] (2) There is currently no transgenic research aimed at increasing the reproductive capacity of male fish

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