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Preparation and application of ricin B

A protein and type 2 diabetes technology, applied in the field of anti-diabetic protein drugs, can solve the problems of many side effects and unsatisfactory effects, and achieve the effects of lowering blood sugar and blood lipids, improving body weight and drinking water

Active Publication Date: 2019-11-05
海木集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problem of unsatisfactory effects and many side effects of current drugs for treating type 2 diabetes, the present invention provides a recombinant ricin B chain protein mutant and its expression method, and verifies its hypoglycemic effect on type 2 diabetes rats

Method used

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  • Preparation and application of ricin B
  • Preparation and application of ricin B
  • Preparation and application of ricin B

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1 Recombinant ricin B chain protein mutant preparation

[0024] 1. Obtaining of Escherichia coli expression engineering strain BL21(DE3) / PET28a-tRTB

[0025] Under the condition that the amino acid sequence remains unchanged, codon optimization is carried out according to the existing ricin B chain (rRTB) mutant, and the entire codon-optimized sequence of the above-mentioned ricin B chain is used as a template, and tRTB sense primer is used PCR amplification was performed with tRTB anti-sense primer, and the obtained PCR product was connected to the pMD19-T simple cloning vector (Treasure Bioengineering (Dalian) Co., Ltd.) to obtain pMD19T-tRTB. Linked with the PET-28a expression vector that was cut with the same NdeI and HindIII restriction enzymes, the resulting linked product was transferred into Escherichia coli competent cells BL21 (DE3), and positive bacteria were obtained by screening, and the plasmids of the positive bacteria were extracted and sequen...

example 2

[0029] Example 2 Recombinant ricin B chain protein mutant anti-type 2 diabetes rat effect experiment

[0030] 1 Experimental animals and grouping

[0031] 1.1 Model making

[0032] The male wistar rats used in the test, 10 were randomly selected as the normal control group, and after the other rats were fed with high-fat diet for 4W, the remaining single small dose (30mg / kg) was intraperitoneally injected with STZ to induce type 2 diabetes model (STZ dissolved in citric acid-sodium citrate buffer solution, injected within half an hour), after 1W, fasting but not water for 12h, blood was taken from the tail vein, and the fasting blood glucose (FBG) was measured with a blood glucose meter, wherein FBG≥7.8mmol / l was a successful model of type 2 diabetes. The experimental animals were fed in a 12-hour standardized feeding room with alternating light and dark in the animal center. Sugar feed, experimental animals free to drink water.

[0033] 1.2 Grouping and administration of...

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Abstract

The invention discloses preparation and application of ricin B, and relates to the technical field of anti-diabetic medicines, in particular to a recombinant protein medicine for treating type 2 diabetes mellitus. The ricin B can reduce the blood sugar and the blood lipid of a bandicoot suffering from the type 2 diabetes mellitus, and can be developed into the medicine for treating the type 2 diabetes mellitus.

Description

technical field [0001] The invention relates to the technical field of antidiabetic protein drugs, in particular to a recombinant ricin B chain protein mutant for treating type 2 diabetes. Background technique [0002] Diabetes mellitus is an immune system disease caused by insufficient insulin secretion and / or insulin resistance, which is a disorder of sugar, fat and protein metabolism. It involves all systems of the body and can even induce many fatal complications, seriously endangering human health. Diabetes has become the current The third major disease that endangers human health. More than 90% of diabetic patients are type 2 diabetes (type 2diabetes mellitus, T2DM). Currently, about 246 million people worldwide suffer from T2DM, and it is estimated that the number of T2DM patients will increase to 380 million within 20 years. China is the country with the largest number of diabetic patients in the world, but the blood sugar compliance rate of Chinese T2DM patients i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/16A61P3/10
CPCA61K38/168A61P3/10A61K38/16C07K14/415
Inventor 刘文森许娜侯峰曹利利宫鹏涛李思明陈星远丁鹤王典
Owner 海木集团有限公司
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