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Optimization method of extraction process of total triterpenes in cyclocarya paliurus and extraction method

A technology of extraction process and optimization method, which is applied to the extraction process optimization method and extraction field of total triterpenes in Cyclocarya paliurus, can solve difficult problems such as the interaction relationship between multiple response values ​​and factors

Inactive Publication Date: 2019-11-08
桑迪(武汉)生物科技有限公司
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Problems solved by technology

[0003] At present, orthogonal design and uniform design are widely used at home and abroad. These two methods are different from comprehensively examining the interaction of various factors, and it is also difficult to analyze the interaction relationship between multiple response values ​​and factors.

Method used

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  • Optimization method of extraction process of total triterpenes in cyclocarya paliurus and extraction method
  • Optimization method of extraction process of total triterpenes in cyclocarya paliurus and extraction method
  • Optimization method of extraction process of total triterpenes in cyclocarya paliurus and extraction method

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Embodiment

[0046] A method for optimizing the extraction process parameters of Cyclocarya paliurus total triterpenoids based on response surface methodology, comprising the following steps:

[0047] (1) Take Cyclocarya paliurus medicinal material, dry to constant weight, pulverize, and pass through a 40-mesh sieve to obtain Cyclocarya paliurus powder for subsequent use;

[0048] (2) Drawing of oleanolic acid reference substance absorbance standard curve

[0049] Take 3.28mg of oleanolic acid reference substance, weigh it accurately, add 5ml of methanol, and shake well to obtain the reference substance solution.

[0050] Precisely measure 0, 0.2, 0.4, 0.6, 0.8, 1.0ml of the reference substance solution, put it in a 10ml test tube, evaporate the solvent, let it cool, and precisely add 0.5ml of the newly prepared 5% vanillin-glacial acetic acid solution, high chlorine Acid 1.4ml, shake well, heat in 70°C water bath for 15min, take out and cool to room temperature, dilute to 10ml with ethyl...

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Abstract

The invention discloses an optimization method of an extraction process of total triterpenes in cyclocarya paliurus and an extraction method. The optimization method includes the steps that influencesof extraction time, material liquid ratio, extraction temperature and ethanol volume fractions on the extraction rate of the total triterpenes are comprehensively considered, a quadratic polynomial equation model of the relationship between the extraction rate of the total triterpenes and the four factors is established by a response surface method, a three-dimensional curved surface diagram is drawn for comprehensive analysis, and optimum process conditions are obtained, wherein a volume fraction of ethanol is 60%, the material liquid ratio is 1 to 30, the extraction time is 180min, the temperature is 72 DEG C and the times of extraction is 3. The verification experiment proves that the process parameters are accurate and reliable; and the response surface method is designed by Box-Behnken and statistical software Design Expert 7.00 is used for simple and reliable optimization tests and data analysis, and good results are obtained. According to the optimization method of the extraction process of the total triterpenes in cyclocarya paliurus and the extraction method, a reference is provided for the evaluation of the inner quality of the cyclocarya paliurus, and a foundation is laid for the promotion of the cyclocarya paliurus.

Description

technical field [0001] The invention belongs to the technical field of natural extracts, and relates to an extraction process optimization method and an extraction method of total triterpenes in Cyclocarya paliurus. Background technique [0002] Cyclocarya paliurus (Batal.) Iljinsk is the leaf of Cyclocarya paliurus (Batal.) Iljinsk, a plant of the family Juglandaceae, also known as money willow and money tree. It is a unique single-species plant in China and one of the endangered plants under national key protection. , Cyclocarya paliurus is a tall fast-growing tree with odd-numbered pinnate compound leaves, distributed in mountainous areas with an altitude of 450-2500 m in Jiangxi, Zhejiang, Guizhou, Guangxi, Hubei and other places; according to the records of "Chinese Traditional Medicine Resources", Its bark and leaves have the effects of clearing heat, detoxifying and relieving pain, and are used to treat stubborn ringworm. Modern research shows that Cyclocarya paliurus...

Claims

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Application Information

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IPC IPC(8): A61K36/52G16C20/70
CPCA61K36/52A61K2236/333A61K2236/39G16C20/70
Inventor 张丹丹甘祥俊熊瑜占密勤冯莎
Owner 桑迪(武汉)生物科技有限公司
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