Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A device for extracting adipose-derived stromal vascular components and its extraction method

A stromal vascular component, fat source technology, applied in the field of biomedicine, can solve the problems of poor SVF activity, the improvement effect needs to be improved, and the time-consuming, etc., to achieve the effect of improving work efficiency

Active Publication Date: 2021-12-10
FOURTH MILITARY MEDICAL UNIVERSITY
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the enzymatic digestion method requires high aseptic conditions and the introduction of exogenous biological enzymes, which takes a long time and has high potential risks. It is mostly used to prepare SVF under laboratory conditions and has not yet been approved for clinical application.
The high-speed centrifugation method is a mechanical preparation method, which is relatively safe, but the activity of the SVF extracted by it is poor, and the improvement effect on fat transplantation still needs to be improved.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A device for extracting adipose-derived stromal vascular components and its extraction method
  • A device for extracting adipose-derived stromal vascular components and its extraction method
  • A device for extracting adipose-derived stromal vascular components and its extraction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] A method for extracting fat-derived stromal vascular components, comprising the following steps:

[0072] In step 1, the tissue fascia of the isolated fat mass is removed and shredded to obtain granules.

[0073] Step 2, add physiological saline to the particulate matter (the physiological saline uses a sodium chloride solution with a concentration of 0.9%, and the dosage is just enough to submerge the particulate matter), and vortex and vibrate for 15 minutes at a frequency of 30 Hz to obtain a suspension.

[0074] In step 3, the suspension was centrifuged at 1000 rpm for 2 min to obtain a centrifuge.

[0075] Step 4: Filter the centrifugate with a 100-mesh filter to obtain the stromal blood vessel components at the bottom of the tube.

Embodiment 2

[0077] A method for extracting fat-derived stromal vascular components, comprising the following steps:

[0078] In step 1, the tissue fascia of the isolated fat mass is removed and shredded to obtain granules.

[0079] Step 2, adding physiological saline (NaCl solution with a concentration of 0.9% for physiological saline, the dosage is enough to submerge the particles) to the particles, and vortexing at a frequency of 50 Hz for 2 minutes to obtain a suspension.

[0080] In step 3, the suspension was centrifuged at 500 rpm for 5 min to obtain a centrifuge.

[0081] Step 4: Filter the centrifugate with a 100-mesh filter to obtain the stromal blood vessel components at the bottom of the tube.

Embodiment 3

[0083] A method for extracting fat-derived stromal vascular components, comprising the following steps:

[0084] In step 1, the tissue fascia of the isolated fat mass is removed and shredded to obtain granules.

[0085] Step 2, add physiological saline to the particulate matter (the physiological saline uses a sodium chloride solution with a concentration of 0.9%, and the dosage is enough to submerge the particulate matter), and vortex and vibrate at a frequency of 5 Hz for 25 minutes to obtain a suspension.

[0086] In step 3, the suspension was centrifuged at 1500 rpm for 1 min to obtain a centrifuge.

[0087] Step 4: Filter the centrifugate with a 100-mesh filter to obtain the stromal blood vessel components at the bottom of the tube.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an extraction device and method for extracting stromal blood vessel components derived from adipose tissue. The extraction device is used for extracting stromal blood vessel components in adipose tissue, and can realize centrifugal movement, vortex movement and oscillation movement of isolated fat mass , centrifugal vibration, and vortex vibration, this method has the advantages of high extraction efficiency, good cell activity, and no introduction of enzyme solution; the various cell components contained in the vascular matrix can be widely used in fat transplantation, cartilage, etc. Repair and other multi-directional fields; the stromal vascular component obtained by vortex oscillation in the extraction method has a high number of viable cells and a strong proliferation ability. The extraction method is simple, low in cost, safe and efficient, and easy to operate and implement.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a device for extracting adipose-derived stromal blood vessel components and an extraction method thereof. Background technique [0002] Adipose tissue is an important regeneration and repair resource in the field of soft tissue repair in recent years. It can be easily obtained through liposuction, and the operation risk is low and the patient is less damaged. Some clinical studies have found that adipose-derived stromal vascular fraction (Stromal Vascular Fraction, SVF) has obvious anti-inflammatory and early vascularization effects. And the field of regeneration has broad prospects. [0003] Currently, SVF is isolated and extracted from adipose tissue mainly by enzymatic digestion or high-speed centrifugation. Among them, the enzymatic digestion method requires high aseptic conditions and the introduction of exogenous biological enzymes, which takes a long time and has high potentia...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36
CPCA61L27/3683A61L27/3633A61L27/3604A61L27/3687A61L27/3691
Inventor 孔亮张凯李云鹏蔡卜磊侯燕刘富伟高晔
Owner FOURTH MILITARY MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com