Lactic acid detection reagent and detection method thereof

Abstract

The invention discloses a lactic acid detection reagent and a detection method thereof. The detection reagent includes LOD, HRP, ABTS and a buffer solution; the configuration range of a lactic acid detection reaction system is that the ratio of an ABTS solution to an LOD solution to an HRP solution is (2.5-5):(1.4-2.6):(2-5). The detection method includes the following steps that the absorbance value is read through a 430 nm spectrum, the absorbance value is read through a microplate reader, a standard curve is made, and then the lactic acid content in samples can be calculated out through thestandard curve. The content of a large amount of lactic acid can be detected quickly and accurately by using a small number of samples, and meanwhile, the cost of unit sample detection is reduced.

Description

technical field [0001] The invention relates to the field of chemical detection, in particular to a lactic acid detection reagent and a detection method thereof. Background technique [0002] Lactic acid, an organic acid with formula C 3 h 6 o 3 . In the process of cell culture, lactic acid mainly comes from the incomplete oxidation of glucose in the metabolic process. When the oxidation is insufficient, excessive NADH accumulates, which starts the glycolysis pathway, and lactic acid is produced accordingly. The accumulation of lactic acid will make the culture system The pH decreases, the activity of intracellular enzymes is affected, which limits the normal growth and metabolism of cells, resulting in acidification and even death of cells. In addition, the lye added to adjust the pH and the high concentration of lactic acid itself will lead to an increase in the osmotic pressure of the system, thereby affecting cell growth, protein production, and quality. In the proc...

AI Technical Summary

Problems solved by technology

Although the instrument detection is highly automated and convenient, it is limited by the throughput of the instrument, it is difficult to complete the detection of a large number of samples at the same time, and it is easily interfered by many coexisting components in the sample

In addition, the use of biochemical analyzers to detect Lactate is limited by the instrument, and supporting reagents, calibration materials and quality control materials are required, and the unit sample cost is relatively high

Although the cost of the ultraviolet method is relatively low, it requires a relatively large amount of sample, which is not conducive to the use of small volume cell culture

The detection cost of the existing technology is relatively high, the required sample volume is large, and a large number of sample detection cannot be completed at the same time, and the use is limited by instruments, consumables, resources, etc.; the market needs a method that can quickly, accurately, and large amounts of lactic acid with a small amount of samples. The detection reagent of content detection, the present invention solves such problem

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