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Gradient density cartilage repair hydrogel and preparation method thereof

A cartilage repair and gradient density technology, applied in the field of medical materials, can solve the problems of scaffolding materials and large open incisions

Active Publication Date: 2021-12-03
JIANGSU DISSUETECH MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the other hand, since the natural cartilage structure is anisotropic and has a gradient structure, the cartilage repair materials in existing patents and literatures are either isotropic, or although they have a gradient structure, their gradient structure has a gradient structure before implantation. Established, i.e. the stent material cannot be implanted by injection and requires a large open incision

Method used

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  • Gradient density cartilage repair hydrogel and preparation method thereof
  • Gradient density cartilage repair hydrogel and preparation method thereof
  • Gradient density cartilage repair hydrogel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Embodiment 1, gradient density type cartilage repair hydrogel raw material composition

[0061] The gradient density water gel raw material composition for cartilage repair in this embodiment is composed of component A and component B, component A is an aldylated polysaccharide as a main chain molecule, and component B is an amine as a crosslinking agent disaccharides. For component A, the aldylated polysaccharide is aldylated hyaluronic acid, aldylated hydroxymethyl chitin, or aldylated dextran; for component B, the aminated disaccharide is aminated sucrose, amine maltose, aminated trehalose and aminated lactose.

[0062] The present embodiment comprises several examples, and the specific compositions of component A and component B of each example are shown in the table below:

[0063] instance number Component A Component B 1 Aldylated Hyaluronic Acid Aminated sucrose 2 aldylated hydroxymethyl chitin Aminated maltose 3 Aldehylated...

Embodiment 2

[0090] Embodiment 2, gradient density type cartilage repair hydrogel

[0091] The gradient density hydrogel for cartilage repair of this embodiment is prepared from the raw material composition of the gradient density hydrogel for cartilage repair in Example 1 through the following steps:

[0092] U1. Dissolve component A in buffer to form solution A, and dissolve component B in buffer to form solution B; the concentration of component A in solution A is 5-20 mg / mL; The concentration of B is 5-20 mg / mL; the buffer is PBS buffer; the concentration of component A in solution A is the same as that of component B in solution B.

[0093] Note: In actual use, after step U1, pass each solution through a 0.22μm filter head to sterilize, and then fill them in 5mL pre-filled glass syringes under a sterile environment for use.

[0094] U2. Blend solution A with solution B; the volume ratio of solution A to solution B is 2:1.

[0095] Note: When implementing joint repair, inject the mix...

Embodiment 3

[0101] Embodiment 3, animal joint repair experiment

[0102] Experimental animals: 6 groups of SPF grade New Zealand white rabbits, 3 in each group, male, 6-8 months old, 2-2.5kg.

[0103] Operation process: each group of rabbits corresponds to one example of embodiment 2. 3% sodium pentobarbital was anesthetized along the rabbit's ear vein at 1㎎ / ㎏. After the anesthesia was successful, under aseptic operation, the rabbit was placed in a supine position, the skin of the left knee joint was prepared, the skin was cut in the middle of the knee joint, and the inner side of the patella was cut Open the knee joint, expose the knee joint, and expose the femoral trochlear after flexing the knee joint. A defect with a diameter of 3.5 mm and a depth of 3.0 mm was made in the femoral trochlea using cartilage grafting instruments. Inject the mixed gel into the defect according to the following steps: Take the solution A and solution B filled after the U1 step of each example in Example ...

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Abstract

The invention relates to a gradient density type hydrogel for cartilage repair and a preparation method thereof. The hydrogel is prepared from solution A and solution B. The component A contained in the solution A is an aldylated polysaccharide as a main chain molecule, and the component B contained in the solution B is an aminated disaccharide as a crosslinking agent. The hydrogel prepared by the preparation method has a cross-linked network with a gradient distribution of dense up and sparse down along the direction of gravity, which can simulate the gradient density structure of natural cartilage. The hydrogel of the present invention can form a gel in situ at the joint defect through minimally invasive injection, and the formed gel can simulate the gradient density structure of natural cartilage, which is dense at the top and sparse at the bottom; it can be used in conjunction with microfracture Guide autologous stem cells to differentiate into a multi-layer orientation structure similar to cartilage to achieve a truly complete repair of joint defects.

Description

technical field [0001] The invention relates to a gradient density hydrogel for cartilage repair and a preparation method thereof, belonging to the technical field of medical materials. Background technique [0002] As far as the applicant knows, biomedical materials are often used to repair or replace part or all of the natural functions of the human body structure. Many biomedical materials are used as medical devices or components of devices, such as internal tissue glue, surgical hemostatic materials, anti-adhesion materials, tissue repair and skin substitutes, or drug-eluting stents that can release drugs. Hyaluronic acid, chitosan, collagen, sodium alginate, etc. are all biomedical materials with a wide range of clinical applications. [0003] Tissue engineering repair is very common in clinical work, among which, articular cartilage repair has always been a clinical problem. Unlike bones, articular cartilage lacks blood vessels, nerves, and lymphatic systems. Once t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/20A61L27/50A61L27/52A61K9/06A61K47/36C08J3/075C08L5/08C08L5/02
CPCA61K9/06A61K47/36A61L27/20A61L27/50A61L27/52A61L2430/06C08J3/075C08J2305/02C08J2305/08C08L5/08C08L5/02
Inventor 万文倩曹彬徐兴全朱健
Owner JIANGSU DISSUETECH MEDICAL TECH CO LTD