Metarhizium anisopliae strain and application thereof

A technology of Metarhizium anisopliae and Metarhizium anisopliae, applied in the fields of application, fungi, and treatment of microorganisms with electricity/wave energy, can solve the problems of human and animal health hazards, environmental pollution, etc., and achieve the effect of strong insecticidal ability

Pending Publication Date: 2019-11-12
INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical pesticide poison bait has a good control effect, but it is easy to cause environmental pollution and cause harm to human and animal health

Method used

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  • Metarhizium anisopliae strain and application thereof
  • Metarhizium anisopliae strain and application thereof
  • Metarhizium anisopliae strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A strain of Metarhizium anisopliae of the present invention, the strain is Metarhizium anisopliae JXAS-03; the preservation name is Metarhizium anisopliae JXAS-03; it is preserved in China Typical Culture Collection Center, and the preservation address is Wuhan City, Hubei Province China Type Culture Collection Center of Wuhan University, Bayi Road, Hongshan District; date of preservation: August 14, 2019; preservation number: CCTCC M 2019626.

[0028] Metarhizium anisopliae JXAS-03 on the PDA plate, the initial vegetative growth stage is white, small filamentous fungus, grows radially outward, and the fungus becomes green or yellow-green in the later stage of sporulation, and the center of the colony is occasionally dotted of white.

[0029] The nucleotide sequence of the described Metarhizium anisopliae is shown in SEQ ID NO:1.

[0030] The Metarhizium anisopliae bacterial agent prepared by the Metarhizium anisopliae of the present invention. Its active ingredient i...

Embodiment 2

[0040] The difference between embodiment 2 and embodiment 1 is: the preparation method of scarab metarhizium anisopliae bacterial agent of the present invention, comprises the steps:

[0041] In step (1), single colony of bacterial strain is placed on the PDA plate containing the chloramphenicol of 25 μ g / ml, and temperature is 25 ℃, culture 14 days, until produce green spore, wash spore with 0.05% Tween-80, make Obtain Metarhizium anisopliae spore suspension bacterial agent;

[0042] In step (2), or pick a single colony in (1), inoculate it into LB culture solution containing 25 μg / ml chloramphenicol, at a temperature of 25°C, at a stirring rate of 100 rpm, and culture on a shaker for 3 days , preparing the fermentation culture of the Metarhizium anisopliae;

[0043] In step (3), after the fermentation culture in (2) is ultrasonically disrupted, the lysate is centrifuged at 3000 rpm / min, and the precipitate is the metarhizium anisopliae cell ultrasonic lysis precipitate; the...

Embodiment 3

[0045] The difference between embodiment 3 and embodiment 1 is: the preparation method of scarab metarhizium anisopliae bacterial agent of the present invention, comprises the steps:

[0046] In step (1), single colonies of the bacterial strain are placed on a PDA plate containing 25 μg / ml of chloramphenicol, and the temperature is 28° C., cultivated for 10 days until green spores are produced, and the spores are washed with 0.05% Tween-80 to prepare Obtain Metarhizium anisopliae spore suspension bacterial agent;

[0047] In step (2), or pick a single colony in (1), inoculate it into LB culture solution containing 25 μg / ml chloramphenicol, at a temperature of 28°C, at a stirring rate of 150 rpm, and culture on a shaker for 8 days , preparing the fermentation culture of the Metarhizium anisopliae;

[0048] In step (3), after the fermentation culture in (2) is sonicated, the lysate is centrifuged at 4000rpm / min, and the precipitate is the metarhizium anisopliae cell ultrasonic ...

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Abstract

The present invention discloses a Metarhizium anisopliae strain and its application. The present invention relates to a Metarhizium anisopliae strain, and the strain is Metarhizium anisopliae; a preservation name is Metarhizium umaniensis (JXAS-03), the strain is preserved at the China Type Culture Collection Center, and a preservation address is China Type Culture Collection Center of Wuhan University, Bayi Road, Hongshan District, Wuhan, Hubei Province; a preservation number is August, 14th, 2019; and a preservation number is CCTCC M 2019626. The strain of the present invention has a stronginsecticidal ability against Chinese mole cricket pests, and provides a new material for the control of Chinese mole cricket pests.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a strain of Metarhizium anisopliae and an application thereof. Background technique [0002] Mole crickets belong to Orthoptera mole crickets, commonly known as Lala cricket, Dila cricket, common mainly include Oriental mole cricket, North China mole cricket and so on. Mole crickets mainly dig tunnels under the soil through adults, feed on plant roots and tender stems, and indirectly cause the roots and soil of plants to detach, causing plant seedlings to dry up. Mole crickets like to dig tunnels in soft, moist soil. Mole crickets are omnivorous insects that harm almost all crops, especially in the seedling and seedling stage of crops, often leading to seedling failure or yield reduction. They are an important class of underground pests, and how to control them is an urgent problem to be solved. [0003] At present, the methods of preventing and controlling mole crickets mainly inc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N13/00A01N63/04A01P7/04C12R1/645
CPCC12N1/14C12N13/00C12R2001/645C12N1/145
Inventor 占智高刘卓荣关丽梅况文东王金昌靳亮李鹏邱小忠张婷
Owner INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI
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