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Rapid screening method of bacillus subtilis fresh-keeping strain

A Bacillus subtilis and screening method technology, applied in the field of rapid screening of Bacillus subtilis fresh-keeping strains, can solve problems affecting the flavor of aquatic products, hidden dangers to human health and safety, and chemical substances affecting flavor, etc.

Active Publication Date: 2019-11-15
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the preservation of aquatic products is mainly through low-temperature preservation and chemical preservation. Too low temperature will affect the original flavor of aquatic products. Although the application of chemical anti-corrosion preservation technology effectively prolongs the shelf life of aquatic products, some chemical substances It will react with fish to affect its flavor, and there are hidden dangers to human health and safety

Method used

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  • Rapid screening method of bacillus subtilis fresh-keeping strain
  • Rapid screening method of bacillus subtilis fresh-keeping strain
  • Rapid screening method of bacillus subtilis fresh-keeping strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 16 strains of Bacillus subtilis (numbered BSS01-BSS16) isolated from tilapia intestines and ponds were randomly selected, and 4 cell adhesion indexes and 2 antibacterial indexes of each strain were detected.

[0035] 1. The hydrophobicity, self-aggregation rate, co-aggregation rate, biofilm formation ability, antibacterial activity and supernatant antibacterial activity of 16 strains of Bacillus subtilis are as follows:

[0036] (1) Hydrophobicity: The surface hydrophobicity of Bacillus subtilis was determined by referring to the microbial adhesion hydrocarbon method, and the fermentation broth was taken after overnight culture and centrifuged to resuspend the bacterial sediment to prepare the bacterial suspension, so that the bacterial suspension was OD 600nm =1.0 ± 0.05. Pipette 2 mL of each bacterial suspension and 2 mL of xylene to mix, vortex for 120 s, and stand at room temperature for 30 min to separate layers. Take 1mL of the aqueous phase and measure its OD 6...

Embodiment 2

[0057] Using the screening equation established in Example 1, Bacillus subtilis with high fresh-keeping activity was rapidly screened.

[0058] The other 6 strains of Bacillus subtilis A01, A06, A03, A11, A12 and A09 screened from tilapia intestines and ponds were used to carry out the back substitution verification test of the equation. The hydrophobicity, self-aggregation rate, co-aggregation rate, biofilm formation ability, fermentation supernatant bacteriostasis rate and spot bacteriostasis rate of these 6 strains were measured. The results are shown in Table 4.

[0059] These 6 indicators of hydrophobicity, self-aggregation rate, co-aggregation rate, biofilm formation ability, fermentation supernatant bacteriostasis rate and spot seed bacteriostasis rate of these 6 strains of bacteria are substituted into the screening equation of embodiment 1, and the comprehensive score 46.40%, 43.69%, 33.58%, 26.15%, 15.60%, and 19.63%, respectively, and A01 and A06 strains were high f...

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Abstract

The invention discloses a rapid screening method of bacillus subtilis fresh-keeping strains. The method comprises the following steps: establishing a rapid screening equation of bacillus subtilis fresh-keeping strains by utilizing four cell adhesion related indexes including hydrophobicity, a self-coagulation rate, a co-coagulation rate and a biofilm forming ability and two antibacterial indexes including a fermentation supernatant antibacterial rate and a dibbling antibacterial rate; y is a sum of 0.151A, 0.285B, 0.029C, 0.207D, 0.184E, and 0.178F, wherein Y is a comprehensive score, A is theself-coagulation rate, B is the hydrophobic rate, C is the co-coagulation rate, D is the biofilm formation rate, E is the fermentation supernatant antibacterial rate, and F is the dibbling antibacterial rate, wherein when Y is greater than or equal to 0 and less than 20%, the bacillus subtilis is low in fresh-keeping activity, when Y is greater than 20% and less than 40%, the bacillus subtilis ismedium in fresh-keeping activity, and Y is greater than or equal to 40% and less than or equal to 100%, the bacillus subtilis is high in fresh-keeping activity. The bacillus subtilis strain with highfresh-keeping activity can be quickly screened through the equation.

Description

technical field [0001] The invention relates to a rapid screening method for fresh-keeping strains of Bacillus subtilis. Background technique [0002] my country is the country with the largest production of aquatic products in the world. Fish farming is the main aquatic economy in my country. How to effectively maintain the freshness of aquatic fish and extend the shelf life is an important issue restricting the development of my country's aquaculture industry. Aquatic products are rich in protein and other nutrients, which can easily cause corruption of fish, lead to deterioration of aquatic products, and even affect water quality and human health and safety. Therefore, research on aquatic product preservation technology is a current research hotspot. [0003] At present, the preservation of aquatic products is mainly through low-temperature preservation and chemical preservation. Too low temperature will affect the original flavor of aquatic products. Although the applic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12Q1/18
CPCC12Q1/04C12Q1/18
Inventor 张雯游佳鸿倪莉
Owner FUZHOU UNIV
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