African swine fever virus antigen duplex detection reagent and preparation method thereof

Abstract

The invention discloses an African swine fever virus antigen duplex detection reagent and a preparation method thereof. The African swine fever virus antigen duplex detection reagent comprises a PVC bottom plate, wherein a sample pad, a colloidal gold marking pad, a nitrocellulose membrane detection pad and a water absorption pad are arranged at the top of the PVC bottom plate from left to right in sequence. According to the African swine fever virus antigen duplex detection reagent disclosed by the invention, by means of the detection of main and fairly conserved antigenic two proteins (p30 and p72) of AFSV, two results can be simultaneously obtained by once sample injection, furthermore, AFSV infection can be prompted as long as any one of the two results is positive, so that the detection rate is improved, whether early pig fever is caused by African swine fever virus infection can be quickly detected so as to discover the epidemic situation and take effective prevention and controlmeasures, furthermore, the African swine fever virus antigen duplex detection reagent has high sensitivity, high specificity, high speed (5-10min) ), simple and convenient operation, good repeatability, stable structure, good use performance, and is suitable for farmers to carry out self-examination or for professional institutions to carry out investigation of epidemic situation.

Description

technical field [0001] The invention relates to the technical field of virus detection, in particular to an African swine fever virus antigen double detection reagent and a preparation method thereof. Background technique [0002] African swine fever is caused by African swine fever virus. It mainly infects pigs such as domestic pigs, wild boars, and bush pigs. It has a high mortality rate and causes huge economic losses to the pig industry. ASFV is the only known A DNA arbovirus. ASFV only infects domestic pigs and wild boars, but not humans. Wild boars are usually recessively infected after ASFV infection. In addition, soft ticks are also the host and transmission medium of ASFV. [0003] The clinical features of pigs infected with ASFV are high fever, diarrhea, hemorrhage and skin flushing. Some clinical symptoms and autopsy results, such as splenomegaly, lymph nodes and kidney hemorrhage, are indistinguishable from the symptoms of classical swine fever virus infection in...

AI Technical Summary

Problems solved by technology

[0004] At present, the main reagents for detecting African swine fever are all dependent on imports. However, due to the relatively complicated ELISA operation method, negative and positive controls are required each time, and the detection is relatively cumbersome. Therefore, we propose a dual detection of African swine fever virus antigen Reagent and its preparation method

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