Multiplex fluorescence quantitative PCR kit for detecting 19 human respiratory viruses

A multiple fluorescence quantitative and virus detection technology, applied in the biological field

Pending Publication Date: 2019-11-19
厦门安普利生物工程有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no detection of 19 pathogens (virus + mycoplasma/chlamydia) for viral respiratory tract infection.

Method used

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  • Multiplex fluorescence quantitative PCR kit for detecting 19 human respiratory viruses
  • Multiplex fluorescence quantitative PCR kit for detecting 19 human respiratory viruses
  • Multiplex fluorescence quantitative PCR kit for detecting 19 human respiratory viruses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0107] Example 1: Preparation of a multiplex fluorescent quantitative PCR kit for the detection of 19 human respiratory viruses

[0108] The present invention is used for the detection of 19 kinds of human respiratory tract viruses multiplex fluorescent quantitative PCR kit comprises PCR amplification kit and reference product kit, wherein, the composition of PCR amplification kit is shown in Table 1, and the composition of reference product kit is shown in Table 1 2.

[0109] Table 1 Composition list of PCR amplification kit

[0110]

[0111] Table 2 List of components of the reference kit

[0112]

[0113] The kit of the present invention can be used to detect 19 kinds of human respiratory viruses: influenza A virus (IAV), influenza B virus (IBV), human parainfluenza virus type Ⅰ (HPIVⅠ), human parainfluenza virus type Ⅱ (HPIVⅡ), Human parainfluenza virus type Ⅲ (HPIVⅢ), human adenovirus (HADV), human respiratory syncytial virus (HRSV), human coronavirus 229E group (...

Embodiment 2

[0153] Embodiment 2: the detection method of multiplex fluorescent quantitative PCR kit

[0154] 1. Detection Kit

[0155] The multiplex fluorescence quantitative PCR kit prepared in Example 1 for the detection of 19 kinds of human respiratory viruses.

[0156] Detection object

[0157] 2.1 Preparation of internal standard granular saline containing the housekeeping gene β-actin in human cells (hereinafter referred to as "internal standard saline"):

[0158] In the reference substance preparation room in the clean work area, measure 800mL of normal saline (0.9% sodium chloride solution) into a 1000mL volumetric flask, add 5.0×10 9 Copies / mL pseudovirus particles (RNA virus) 1mL, dilute to 1000mL with normal saline, turn over to mix well, so that the final concentration is 5.0×10 6 copies / mL. Transfer the above solution to a 1000mL beaker and divide it for use.

[0159] 6 samples tested negative

[0160] Collect respiratory tract samples such as Staphylococcus aureus, St...

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PUM

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Abstract

The invention relates to a multiplex fluorescence quantitative PCR kit for detecting 19 human respiratory viruses. The multiplex fluorescence quantitative PCR kit comprises a PCR amplification kit anda reference kit, wherein the PCR amplification kit comprises RVD19 amplification solutions 1#-8# . The kit adopts an 8-hole combined detection method, wherein each hole comprises three fluorescence channels, and each channel corresponds to one pathogen, so that the 19 human respiratory viruses can be qualitatively detected and distinguished simultaneously, which is currently the most comprehensive viral respiratory tract infection pathogen detection method, and provides a basis for comprehensive judgment of the viral respiratory tract infection pathogens and laboratory-assisted diagnosis forclinical examination.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a multiplex fluorescent quantitative PCR kit for detecting and distinguishing 19 kinds of human respiratory viruses, and a detection method of the kit. Background technique [0002] Viral respiratory tract infection is divided into viral upper respiratory tract infection and viral lower respiratory tract infection. The former manifests as acute rhinitis, pharyngitis and laryngitis, and the latter manifests as acute tracheitis, bronchitis and pneumonia. The incidence of this type of disease is high, and the crowd is generally susceptible to infection. Children, the elderly, malnourished and those suffering from chronic diseases are more susceptible to the disease. It is more common in winter and spring. The disease can come on in all seasons and at any age, and it can be transmitted by droplets containing virus, droplets, or contaminated utensils. Often when the body's resistance is...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/689C12Q1/04C12Q1/6851C12R1/93C12R1/35
CPCC12Q1/701C12Q1/689C12Q1/6851C12Q2600/16C12Q2600/166C12Q2531/113C12Q2537/143C12Q2545/101C12Q2563/107
Inventor 陈泽斌王伟毅魏超魏劭
Owner 厦门安普利生物工程有限公司
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