Sterile culture device for endophyte inoculation experiment through root irrigation method

Abstract

The invention discloses a sterile culture device for an endophyte inoculation experiment through a root irrigation method. The device comprises a cylindrical device body and a drip irrigation device.A cover body is arranged at the upper portion of the device body, a bottle opening is arranged in the middle of the cover body, a filtration bacteriological breathable film is arranged at the bottle opening, an opening is formed in the device body, a stretchable device is arranged at the opening, and the drip irrigation device is communicated with the device body through the stretchable device. The plant sterile culture device can be repeatedly used after cleaning and sterilization; irrigation and inoculation at the roots of plants are easy, the irrigation position and the irrigation liquid amount can be conveniently controlled according to the growth position, accurate irrigation and inoculation can be performed at the roots of the cultured roots; the sterile state inside a culture bottlecan be kept, the influence of the experiment host plant biological effect on other microorganisms can be lowered to the maximum extent, and the real effect of endophytes is reflected.

Description

technical field [0001] The invention relates to the technical field of experimental devices, in particular to an aseptic culture device for inoculating plant endophytic bacteria by root irrigation and inoculation. Background technique [0002] Plant endophytes are fungi or bacteria that live in the tissues and organs of healthy plants for some or all stages. Plant endophytes are fungi that can not only produce various chemical substances, but also inhibit or kill various pathogenic bacteria through competition or other effects, and do not cause obvious disease symptoms to plant tissues, including those in the life cycle. Saprophytic bacteria that grow on the surface of the camp at a certain stage, latent pathogenic bacteria and mycorrhizal fungi that are temporarily harmless to the host, etc., can be isolated from several to dozens of endophytic bacteria on one plant. Plant endophytes are a new microbial resource with great potential application value. [0003] The traditi...

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Problems solved by technology

In the process of artificially inoculating endophytic bacteria, in order to avoid the influence of other microorganisms on the experimental endophytic inoculation, it needs to be carried out in a sterile culture environment. The current plant culture device usually has a cover with a filter and a breathable membrane on the top These devices are feasible for the second inoculation method above. This inoculation method only needs to place the treated seeds in the cultivation device at one time, and control the cultivation conditions such as temperature externally until the end of the experiment. There is no need to open the culture device during the process, so as to keep the consistency of the culture environment well. However, the above two inoculation methods need to be inoculated after the leaves grow out, and the existing culture device needs to open the upper cover for manual inoculation. , so the existing culture device is difficult to maintain the consistency of the culture environment, and the experimental process is easily affected by other microorganisms

At present, there are few reports on the special culture device used for this experiment, but this culture device is a device with cutting-edge experimental significance

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