Morus australis root extract as well as extraction separation method and application thereof

A technology of root extract and separation method, which is applied in the field of chicken mulberry root extract, can solve the problems of no report on the application of chicken mulberry root extract, and less research on the chemical composition and activity of the root, and achieve good anti-tumor activity, inhibiting autophagy and promoting cell death

Inactive Publication Date: 2019-11-22
NORTHWEST UNIV(CN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Through literature search to the prior art, it is found that the researches on mulberry mulberry focus on the leaves and stem bark, and ...

Method used

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  • Morus australis root extract as well as extraction separation method and application thereof
  • Morus australis root extract as well as extraction separation method and application thereof
  • Morus australis root extract as well as extraction separation method and application thereof

Examples

Experimental program
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Embodiment 1

[0049] After 7.8kg of dry chicken mulberry root is pulverized, according to the solid-liquid ratio of 1:5 (g / mL), it is 75% ethanol aqueous solution room temperature percolation method to extract 2 times with 39L volume concentration, each extraction time is 48 hours, The extract was concentrated under reduced pressure at 50°C and -0.09Mpa to obtain 0.91kg of extract.

[0050] The separation method of compounds 1-12 in the above-mentioned chicken mulberry root extract is as follows: the extract is subjected to silica gel column chromatography, the chromatography conditions are: 100-200 mesh silica gel, and the volume ratio is 100:1-0:1 dichloromethane - Gradient elution of methanol solution, that is, the volume ratio of dichloromethane-methanol is 100:1, 90:1, 80:1, 70:1, 60:1, 50:1, 40:1, 30:1, The solutions of 20:1, 10:1, 5:1, 1:1, and 0:1 were eluted sequentially to obtain 4 fractions of different polarities, I to IV;

[0051] Fraction II is subjected to silica gel column ...

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Abstract

The invention discloses a Morus australis root extract, and an extraction separation method and application thereof. The extract comprises the following components: cudraflavone B; morusingin L; cudraflavone C; kuwanon C; austraone A; morusin; mulberrofuran G; mulberrofuran F; moracenin B; kuwanon H; morcin M; and cathafuran B. Some of the compounds show good anti-tumor activity, a tumor cell lineis an A549 human lung cancer cell line, the anti-tumor mechanism is that A549 cell apoptosis is induced through an endogenous mitochondrial pathway, and autophagy is inhibited through a PI3k/Akt/mTORpathway to promote cell death.

Description

technical field [0001] The invention relates to the technical field of plant extraction and separation, in particular to a chicken mulberry root extract, an extraction and separation method and an application thereof. Background technique [0002] Today, with the rapid development of medical science and technology in the world, people are facing more and more diseases. It is still difficult to achieve the effect of curing many diseases that threaten human life. Under this severe situation, medical workers are committed to developing more mild and effective treatments. Treatment methods and drugs with better effects have also achieved certain results. However, the clinical application of chemically synthesized drugs can no longer meet the treatment requirements, and more urgently, the side effects they bring to the human body have also been paid more and more attention. Natural medicines have a long history of use and play an important role in the history of human diseases. ...

Claims

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Application Information

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IPC IPC(8): C07D493/04C07D311/30C07D493/14C07D493/06C07D307/80A61P3/10A61P17/00A61P25/00A61P25/28A61P35/00
CPCA61P3/10A61P17/00A61P25/00A61P25/28A61P35/00C07D307/80C07D311/30C07D493/04C07D493/06C07D493/14
Inventor 白乃生郭森张姗姗岳文平杨闯高兵郭雅欣
Owner NORTHWEST UNIV(CN)
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