Gene, gene fragment, corresponding dsRNA for regulating synthesis of sex pheromone of Blattella germanica and preparation method and application thereof

A technology of German cockroaches and gene fragments, applied in the field of dsRNA and its preparation, to achieve the effects of reducing content and release, good application potential, and reducing protein expression
CN110484539AActive Publication Date: 2019-11-22GUANGMEIYUAN R & D CENT KEY LAB OF INSECT DEVELOPMENTAL BIOLOGY & APPL TECH SOUTH CHINA NORMAL UNIV MEIZHOU +1

Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGMEIYUAN R & D CENT KEY LAB OF INSECT DEVELOPMENTAL BIOLOGY & APPL TECH SOUTH CHINA NORMAL UNIV MEIZHOU
Publication Date
2019-11-22

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Abstract

The invention discloses a gene, a gene fragment, a corresponding dsRNA that regulates the synthesis of sex pheromone of Blattella germanica and a preparation method and application thereof. The nucleotide sequence of the gene that regulates the synthesis of sex pheromone of Blattella germanica is as shown in SEQ ID No.1. The nucleotide sequence of the gene fragment is as shown in SEQ ID No.3. Introduction of dsRNA synthesized using the fragment as a template into female Blattella germanica can significantly inhibit the expression level of CYP4PC1 gene, leading to the reduction of methyl ketonesex pheromone synthesis. Finally, the courtship behavior of some male individuals of Blattella germanica disappears. The invention provides a potential molecular target for the management strategy based on cockroach mating and reproduction.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a gene for regulating the synthesis of sex pheromone of the German cockroach, a gene fragment, a corresponding dsRNA, a preparation method and application thereof. Background technique

[0002] German cockroach is one of the most common worldwide household pests in the order Blattata. It is small in size and often lives in crevices and corners. It can widely spread a variety of pathogenic microorganisms, carry a large number of allergens and induce asthma, which seriously threatens human health. . Due to the tenacious vitality and amazing fecundity of the German cockroach, the female can lay eggs multiple times after a single mating, and each egg sheath can hatch about 40 nymphs. This super fecundity often makes its population The number has grown rapidly in a short period of time. In addition, German cockroaches are heterophagous and prone to resistance to commonly used pesticides...

Claims

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