Shrna for inhibiting the replication of African swine fever virus and its use

An African swine fever virus, virus technology, applied in the direction of DNA/RNA fragments, viruses, antiviral agents, etc., can solve the problems of inability to induce protective immunity, limited effect, etc., achieve good inhibition, inhibit replication, and increase specific infection Effect
CN111235150BActive Publication Date: 2020-10-27苏州沃美生物有限公司
5 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
苏州沃美生物有限公司
Publication Date
2020-10-27

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The present invention discloses a class of shRNAs used for inhibiting replication of African swine fever viruses. The shRNAs contain two shRNA sequences such as SEQ ID NO.1 and SEQ ID NO.2. The present invention also provides a use of the aforementioned shRNAs in inhibiting the replication of the African swine fever viruses. The present invention also provides a recombinant expression vector containing the aforementioned shRNAs, and an adeno-associated virus (AAV) and a preparation method thereof. The provided shRNAs and recombinant AAV can be used to prepare drugs for preventing and / or treating African swine fever virus infection, such as therapeutic vaccines and can also effectively inhibit the replication and infection of the African swine fever viruses, and the preparation process is simple and conducive to large-scale production and promotion and application.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The present invention relates to a kind of shRNA (short hairpin RNA), in particular to a kind of shRNA for inhibiting the replication of African swine fever virus and its application, such as the application in preparing African swine fever virus vaccine, which belongs to the technical field of bioengineering. Background technique

[0002] RNA interference (RNA interference, RNAi) refers to the phenomenon that double-stranded RNA molecules (dsRNA) enter human cells and specifically degrade their homologous mRNAs, thereby specifically and efficiently inhibiting the expression of corresponding genes (Waterhouse, P.M., Wang, M.B. , Lough, T., (2001). Gene silencing as an adaptive defense against viruses. Nature 411:834–842.). When a homologous dsRNA with the coding region of endogenous mRNA is introduced into cells, the mRNA will be degraded and gene expression will be silenced, which is a special post-transcriptional gene silence (PTGS). Therapeutically...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More