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Tobacco gene LBM1, screening method thereof and application to controlling plant epidermal hair development

A plant epidermis, gene technology, applied in the field of genetic engineering, can solve the problems of instability, plant leaves falling off, and cumbersome work.

Inactive Publication Date: 2019-11-22
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, plant hormones regulate the development of plant epidermis with low efficiency, high cost, instability, and inability to form genetic stability, resulting in the need for spraying or injection every time, cumbersome follow-up work, and also affect the physiological development process of the plant itself, such as the early fall of plant leaves , precocious fruit, etc.

Method used

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  • Tobacco gene LBM1, screening method thereof and application to controlling plant epidermal hair development
  • Tobacco gene LBM1, screening method thereof and application to controlling plant epidermal hair development
  • Tobacco gene LBM1, screening method thereof and application to controlling plant epidermal hair development

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Embodiment 1

[0035] Tobacco gene LBM1 screening method, comprising the following steps:

[0036] (1) Screening of tobacco gene LBM1

[0037] Extract wild-type tobacco total RNA from tobacco (Nicotiana tabacum cv. GO analysis screened out a key gene related to glandular hair development: LBM1

[0038] (2) Cloning of tobacco gene LBM1

[0039] Using the LBM1 clone forward primer 5'-ATGGTGAGAGCTCCTTGTTG-3' and the LBM1 clone reverse primer 5'-TCAAAATTCCGGTAAATCTAG-3' of AB028649.1 respectively, the target gene was amplified from tobacco by RT-PCR, and the results were as follows figure 1 shown;

[0040] The RT-PCR reaction program is: 50°C for 30min, 94°C for 2min, 94°C for 30S, 55-65°C for 30S, 72°C for 1min / kb, 72°C for 10min, 4°C∞, 30 cycles;

[0041] Obtaining of LBM1 cloning vector: clone the amplified PCR product directly into such as figure 2 The intermediate vector for cloning: on pGEM-T; under the action of T4 DNA ligase, mix the PCR product and the T vector thoroughly, and lig...

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Abstract

The invention discloses a tobacco gene LBM1, a screening method thereof and application to controlling epidermal hair development. The gene has the nucleotide sequence and amino acid sequence shown inAB028649.1, and the tobacco gene LBM1 is applied to controlling the plant epidermal hair development. The plant epidermis development can be controlled, and the plant epidermal hair number can be rapidly and efficiently increased.

Description

technical field [0001] The invention relates to the field of genetic engineering, mainly relates to a tobacco gene LBM1, and also relates to a screening method of the tobacco gene LBM1, and an application of the tobacco gene LBM1 in regulating the development of plant epidermis. Background technique [0002] Epidermal hairs are multicellular structures that protrude from plant epidermal cells, also known as indumentum, as epidermal hairs on the surface of plant tissues, which reduce water loss and regulate plant surface temperature by increasing the thickness of the plant epidermal layer , At the same time, it can protect plants from mechanical damage and insects and other plants. Plant glandular hairs play an important role in plant resistance to adversity stress and plant disease and insect pest defense. According to whether there are secretory glands in the epidermal hairs, the epidermal hairs are divided into secretory gland type and non-secretory gland type. Non-secre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/12A01H6/20
CPCC07K14/415C12N15/8261
Inventor 吴拥军包峻州曹宁
Owner GUIZHOU UNIV