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A Molecular Marker Related to the Traits of Total Teat Number in Pigs and Its Application

A technology of molecular markers and nipples, applied in the determination/testing of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., to achieve high sensitivity and good specificity

Active Publication Date: 2021-02-12
赤峰家育种业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is a lack of research on the GWAS analysis of the traits of total teat number in American Large White pigs, and there are only a small number of GWAS studies on the number of teats in non-American Large White pigs (Tang J, et al. Identification of loci affecting teat number by genome-wide association studies on three pig populations[J].Asian-Australasian journal of animal sciences,2017,30(1):1.;Rohrer G A,etal.Genetic analysis of teat number in pigs reveals some developmental pathways independent of vertebra number and several loci which only affect a specific side[J].Genetics Selection Evolution,2017,49(1):4.;Tan C, etal.Genome-wide association study and accuracy of genomic prediction for teatnumber in Duroc pigs using genotyping-by-sequencing[J] .Genetics SelectionEvolution,2017,49(1):35.; Lopes M S,et al.A genome-wide association study reveals dominance effects on number of teats in pigs[J].PloS one,2014,9(8):e105867. ;Duijvesteijn N, et al.High-resolution association mapping of number of teats in pigs reveals regions controlling vertebral development[J].BMCgenomics,2014,15(1):542.)

Method used

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  • A Molecular Marker Related to the Traits of Total Teat Number in Pigs and Its Application
  • A Molecular Marker Related to the Traits of Total Teat Number in Pigs and Its Application
  • A Molecular Marker Related to the Traits of Total Teat Number in Pigs and Its Application

Examples

Experimental program
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Embodiment 1

[0029] Example 1: Genotyping Detection

[0030] (1) Using the magnetic bead method genome extraction kit to automatically extract the total DNA from tissue samples of American Large White pigs (from the experimental pig farm of Huazhong Agricultural University), the specific steps are as follows:

[0031] (1) Take an appropriate amount of tissue samples into a 1.5mL centrifuge tube;

[0032] (2) Add 500 μL of lysate and 5 μL of proteinase K (20 mg / mL) to the centrifuge tube, shake and mix for 30 seconds, place in a 65°C oven or metal bath, and lyse for 30 minutes to 1 hour;

[0033] (3) After the lysis is completed, transfer all the supernatant to a deep-well plate (marked as ①), and add 350 μL of isopropanol to each well;

[0034] (4) Put the No. ① deep-well plate on station 1 of the nucleic acid extractor, and place the deep-well plates with magnetic beads, washing solution ①, washing solutions ② and ③ and eluent on station 2~ 6 on. Turn on the power of the instrument, an...

Embodiment 2

[0042] Example 2: Application of the H3GA0022648 molecular marker typing method in the association analysis of the total teat number traits of American Large White pigs

[0043] (1) Phenotypic pretreatment of traits of total teats in American Large White

[0044] The total teat traits of American Large White pigs need to use the mixed linear model (Mixed Linear Model, MLM), the specific steps are: use the joint effect of field, birth year and birth season, gender, and birth order as fixed effects, and individual effects as random Effect, the estimated breeding values ​​(Estimated breeding values, EBV) of individuals with phenotype records estimated by the model are added to the corresponding estimated residuals (residuals), as the corrected phenotype values ​​(Corrected phenotypes,) of these individuals, using for subsequent GWAS analysis. The DMUAI module under the DMU statistical environment was used for Restricted Maximum Likelihood estimation (Restricted Maximum Likelihoo...

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Abstract

The invention belongs to the technical field of molecule marker screening for pigs, and particularly relates to a molecular marker related to the total teat number character of a pig and application.The molecular marker can be applied to marker-assisted selection for the total teat number character of the pig. According to the molecular marker and the application, genetic typing is conducted through a gene chip technology, the molecular marker related to the total nipple number character of the pig is obtained through screening, and a nucleotide sequence of the molecular marker is shown in SEQ ID NO:1 or is a gene fragment shown in figure 2; A / G allelic mutation exists at the basic group at the 101 position of the sequence and causes polymorphism generation by the nucleotide sequenceof the molecular marker, and when the nucleotide at the 101bp position of the sequence is A, it is judged that the pig has the character of more total teats. According to the molecular marker and theapplication, the significantly-related SNP molecular marker is provided for marker-assisted selection for the total teat number character of the pig.

Description

technical field [0001] The invention belongs to the technical field of porcine molecular marker screening, and in particular relates to a molecular marker related to the trait of total teat number of pigs and its application. The molecular marker can be used for marker-assisted selection of the total teat number trait of pigs. Background technique [0002] The teat number trait is an important trait that affects the reproductive performance of sows and the survival of piglets (Wang L, et al.Genotyping by sequencing reveals a new locus for pig teat number[J].Animalgenetics,2017,48(4):470-472.) . In traditional pig breeding, the selection of traits is mostly focused on litter size traits, such as total litter size, live piglets, 5-day surviving piglets, and 21-day weaned piglets. In the past ten years, traditional breeding methods have continued to use high-intensity selection for litter size, which has resulted in a significant increase in litter size. As of 2013, the aver...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 项韬郭猛余梅赵书红李新云邓大栋梅全顺陈毅龙
Owner 赤峰家育种业科技有限公司