Cordyceps militaris-mung bean bidirectional liquid fermentation process

A technology of liquid fermentation and Cordyceps militaris, which is applied in cosmetics, skin care preparations, medical preparations containing active ingredients, etc., can solve the problem of less application of cosmetics, improve antioxidant and anti-inflammatory activities, and improve unpleasant odors Effect

Inactive Publication Date: 2019-12-03
北京京瑜科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, in some related technologies of combined fermentation of Cordyceps militaris and mung bean, most of them are applied to solve technical problems in food and health care products, such as CN201210254867.0: Relating to a kind of Cordyceps militaris health care product containing Chinese medicine extract and its preparation method and CN201310229581.1: A method for producing fermented cereals from Cordyceps militaris by solid-state fermentation, but it is rarely used in cosmetics

Method used

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  • Cordyceps militaris-mung bean bidirectional liquid fermentation process
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  • Cordyceps militaris-mung bean bidirectional liquid fermentation process

Examples

Experimental program
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Effect test

Embodiment 1

[0040] A kind of Cordyceps militaris-mung bean two-way liquid fermentation process, comprises the following steps:

[0041] S1, configure liquid culture medium

[0042] The mung bean mixture with a solid-liquid ratio of 1:30 was extracted at 70°C for 1.5h, filtered, and the filtrate was obtained to obtain a mung bean extract, and then 60g of glucose, 4.5g of MgSO4, and 1.5g of KH2PO4 were weighed and mixed evenly, and then Add 3L mung bean extract to dissolve, add an appropriate amount of defoamer, assist ultrasound, stir for 3 minutes to promote the dissolution of the medium, and obtain a liquid medium.

[0043] S2, fermentation culture

[0044] Sterilize the cleaned 5L fermenter, test the air tightness of the equipment at the same time, and calibrate the pH and DO (dissolved oxygen) electrodes. After the calibration, transfer the liquid medium to the fermenter, insert the electrodes, and remove them. Wrap the lead wire and the electrode head with tinfoil paper, connect the...

Embodiment 2

[0048] A kind of Cordyceps militaris-mung bean two-way liquid fermentation process, comprises the following steps:

[0049]S1, configure liquid culture medium

[0050] Extract the mung bean mixture with a solid-liquid ratio of 1:10 at 50°C for 1 hour, filter, and take the filtrate to obtain the mung bean extract, then weigh 40 g of glucose, 4 g of MgSO4, and 1 g of KH2PO4 and mix well, then add 2 L of mung bean The extract was dissolved, an appropriate amount of antifoaming agent was added, assisted by ultrasound, and stirred for 3 minutes to promote the dissolution of the medium to obtain a liquid medium.

[0051] S2, fermentation culture

[0052] Sterilize the cleaned 5L fermenter, test the air tightness of the equipment, and calibrate the pH and DO (dissolved oxygen) electrodes. After the calibration, transfer the liquid culture medium to the fermenter, insert the electrodes, and remove them. Wrap the lead wire and the electrode head with tinfoil paper, connect the pipeli...

Embodiment 3

[0056] A kind of Cordyceps militaris-mung bean two-way liquid fermentation process, comprises the following steps:

[0057] S1, configure liquid culture medium

[0058] Extract the mung bean mixture with a solid-liquid ratio of 1:20 at 60°C for 2 hours, filter, and take the filtrate to obtain the mung bean extract, then weigh 50g of glucose, 5g of MgSO4, 2g of KH2PO4 and mix them evenly, then add 2.5L The mung bean extract was dissolved, and an appropriate amount of defoamer was added, assisted by ultrasound, and stirred for 3 minutes to promote the dissolution of the medium to obtain a liquid medium.

[0059] S2, fermentation culture

[0060] Sterilize the cleaned 5L fermenter, test the air tightness of the equipment, and calibrate the pH and DO (dissolved oxygen) electrodes. After the calibration, transfer the liquid culture medium to the fermenter, insert the electrodes, and remove them. Wrap the lead wire and the electrode head with tinfoil paper, connect the pipeline, w...

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Abstract

The invention discloses a cordyceps militaris-mung bean bidirectional liquid fermentation process. The fermentation process comprises a formula with the following raw material proportion: (1:10) to (1:50) of mung bean mixed liquid, 40-80 g of glucose, 4-6 g of MgSO4 and 1-3 g of KH2PO4, and comprises the following steps that S1, a fluid nutrient medium is prepared, the mung bean mixed liquid withthe material liquid ratio of (1:10) to (1:50) is extracted at 50-90 DEG C for 1-3 h, filtering is conducted, filter liquid is taken to obtain mung bean extract liquid, and then 40-80 g of the glucose,4-6 g of the MgSO4 and 1-3 g of the KH2PO4 are weighed and mixed evenly, then 2-4 L of the mung bean extract liquid is added for dissolving, appropriate defoamers are added, assist ultrasound is conducted, and stirring is conducted for 3 minutes to promote the dissolution of the medium to obtain the fluid nutrient medium; and S2, fermentation and culturing are conducted; and S3, cordyceps militaris mung bean fermentation liquid is collected. The unpleasant smell of fermentation liquid produced by using the traditional nitrogen source is improved, compared with the commercially available cordyceps militaris link, and the anti-oxidation and anti-inflammatory activities of a cordyceps militaris mung bean liquid fermentation mycelium are significantly improved. Hair gel prepared by the mung bean cordyceps militaris fermentation liquid has good moisturizing effect on a human body within 0-2 h.

Description

technical field [0001] The invention relates to the technical field of two-way liquid fermentation of Cordyceps militaris and mung bean, in particular to a two-way liquid fermentation process of Cordyceps militaris and mung bean. Background technique [0002] At present, the application of Cordyceps militaris is mostly its fruiting body, and it is mainly added and applied as a functional extract. But because now the wild Cordyceps militaris resources are sharply reduced, and the artificial cultivation of fruiting bodies of Cordyceps militaris is limited by many factors (land, climate, pests and diseases, long growth cycle, etc.), it is difficult to carry out industrialized production on a large scale and extensively Civilian use. Therefore, there is a significant contradiction between people's desire for health care and therapeutic effects of Cordyceps militaris and the shortage of fruiting bodies of Cordyceps militaris. [0003] Mung bean is a plant of the genus Vigna in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61K8/9728A61Q19/00A61Q19/08
CPCA61K8/9789A61K8/9728A61Q19/00A61Q19/08A61K2800/85
Inventor 孙丽丽左锦辉
Owner 北京京瑜科技有限公司
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