A duck-derived Escherichia coli probiotic strain and its screening preparation method and application

Abstract

A duck-derived Escherichia coli probiotic strain disclosed by the invention has a preservation number of GDMCC NO: 60693. The present invention also provides a method for screening and preparing the bacterial strain, including (1) isolation of duck-derived Escherichia coli: (2) screening for drug sensitivity; (3) screening for acid resistance; (4) screening for tolerance to bile salts; ) bile tolerance screening; (6) safety screening; (7) transforming the genome of the screened strains. The invention also discloses the use of the bacterial strain, which can be applied to the preparation of medicines for preventing and controlling duck-derived Escherichia coli infection and Salmonella infection. The advantage of the present invention is that it can improve the antibody level of the duck body, comprehensively evaluate its safety, and is a new drug in the field of preventing and controlling important bacterial intestinal infection diseases in ducks, and proposes new research for the development of this field It also laid a very positive and valuable foundation for the development of this field.

Description

technical field [0001] The invention belongs to the technical field of bacterial strain screening and recombination, in particular to a duck-derived Escherichia coli probiotic strain, a screening preparation method and application thereof. Background technique [0002] There are many serotypes of avian pathogenic Escherichia coli, and the antigen structure is complex. Selecting the dominant serotype strains to make a vaccine to control poultry-origin E. coli disease, often due to the large number of E. coli serotypes, cannot be popularized and used, and the vaccine may also be caused by endotoxin. Adverse reactions. At home and abroad, there are still no vaccine products with good serum cross-protection efficiency, high protection efficiency, and broad spectrum. In factory areas with better production conditions, the prevention and control of bacterial pathogen infection will be combined with comprehensive production management to reduce the occurrence of colibacillosis and...

AI Technical Summary

Problems solved by technology

In factory areas with better production conditions, the prevention and control of bacterial pathogen infection will be combined with comprehensive production management to reduce the occurrence of colibacillosis and the contamination of pathogenic E. coli. However, the prevention and control of colibacillosis in domestic poultry production still mainly depends on The extensive use of antibiotics, this state has led to a series of serious consequences, such as the emergence of a large number of drug-resistant strains, etc.

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