A digital pcr chip

Abstract

The invention provides a digital PCR chip, which comprises a base, a rotating upper cover, a microarray reaction chamber, a sealing layer, a sealing ring, an elastic buckle, a first rotating shaft, a second rotating shaft and a torsion spring. The surface of the microarray reaction chamber is evenly distributed with 40-100 million micropores, which are used to independently separate and carry the reaction reagent system; the rotating upper cover and the base rotate around the rotating shaft to realize sample filling; the rotating upper cover is provided with a sealing layer And the sealing ring is used to isolate and seal the microarray reaction chamber to avoid cross-contamination and evaporation; the elastic buckle and the base rotate mutually around the second shaft, and the compression and sealing are realized by the built-in torsion spring of the second shaft. The digital PCR chip of the invention does not need an external driving device, saves reagents and is simple to operate.

Description

technical field [0001] The invention relates to the technical field of digital PCR, in particular to a digital PCR chip. Background technique [0002] Polymerase chain reaction (PCR) is a molecular biological technology that can amplify DNA fragments in vitro. Digital PCR (dPCR) is a new technology for absolute quantification of nucleic acid molecules. As a control, the copy number of the target molecule can be directly detected. The principle of dPCR is similar to the "divide and conquer algorithm" in computer science. The reagents with low template are distributed into a large number of micropores. After statistical analysis, most of the micropores have no or only one target molecule. The optical detection module count is the initial template amount. Compared with traditional quantitative PCR, dPCR has higher sensitivity, specificity, high tolerance and accuracy. This technology has been widely used in the detection of very small nucleic acid samples, CNV analysis and ge...

AI Technical Summary

Problems solved by technology

In this method, the error is large when generating droplets, which affects the detection results, and the cost is high

[0005] The second is to use a microfluidic chip for detection. The principle of microfluidics is to squeeze the reagent into the chip channel by applying pressure from the outside, but the driving structure is complex, it takes up a lot of space, the cost is high, and the resistance of the fluid channel is large, so it is easy to have gas residues , this method generally requires a large amount of reagents to fill the chip, which causes a great waste of reagents

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