Rapid PCR chip and rapid fluorescent quantitative detector

Abstract

The invention discloses a rapid PCR chip and a rapid fluorescent quantitative detector. The PCR chip comprises a panel and a bottom film, wherein at least one reaction tank is formed in the bottom surface of the panel; the reaction tank does not penetrate through the panel; the two sides of the reaction tank are provided with a sample adding hole and an exhaust hole and communicate with the top surface of the panel; the bottom film is attached to the bottom surface of the panel; and the panel is made of a light-transmitting material. When the PCR chip is arranged on a temperature control module, the bottom film is tightly attached to the temperature control module; a PCR solution in the reaction tank above the bottom film after sample adding is separated from the temperature control moduleby only a layer of bottom film; the refrigerating capacity or the heating capacity of the temperature control module can rapidly penetrate through the bottom film to be conducted to the PCR solution;and compared with a plastic plate, the film is higher in heat conduction speed and more uniform in heat conduction, so that rapid heating or cooling of the PCR solution is realized, and rapid PCR canbe realized. A heating and refrigerating sheet of the rapid fluorescent quantitative detector is horizontally placed; the PCR chip is horizontally placed on the heating and refrigerating sheet; the bottom film is tightly attached to the plane of the heating and refrigerating sheet; and the refrigerating capacity or the heating capacity of the heating and refrigerating sheet can be rapidly and uniformly conducted to the PCR solution.

Description

technical field [0001] The invention relates to the technical field of molecular biology detection, in particular to a fast PCR reaction chip and a fast fluorescence quantitative detector. Background technique [0002] PCR amplification instrument, also known as PCR gene amplification instrument, PCR nucleic acid amplification instrument, polymerase chain reaction nucleic acid amplification instrument, is a kind of amplification instrument for specific DNA using PCR (Polymerase chain reaction, polymerase chain reaction) technology. Instruments and equipment are widely used in medical and biological laboratories, and can be used for the diagnosis of infectious diseases and paternity testing. Based on the three-step principle of PCR reaction, the PCR instrument needs to set three temperature points of denaturation-annealing-extension. In the standard reaction, the double-stranded DNA is denatured at 90~95℃, and then rapidly cooled to 40~60℃, and the primers are annealed. And ...

AI Technical Summary

Problems solved by technology

[0005] The applicant aims to provide a fast PCR reaction chip with a reasonable structure and a fast fluorescence quantitative detector for the shortcomings of the above-mentioned existing production PCR devices, such as large amount of reaction solution, non-concentrated fluorescence reflection, slow and inaccurate heating speed, and complex structure. , PCR chip has fast heat conduction speed, simple structure and easy processing.

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