Method for testing high-pollution background fungi by separation and purification

Abstract

The invention discloses a method for testing high-pollution background fungi by separation and purification. A double-sided forward fungi purification and separation culture method is adopted, according to a double-sided forward separation method, two culture media are arranged in one culture dish, air is taken as a separation medium, aerial hyphae are induced to grow towards a target culture layer through allelochemicals, or spores are induced to be produced through a feeding layer, and fungi cysts are used for cracking and ejecting spores to separate and purify single spores. The biologicalsafety can be improved, the experiment time can be shortened, the experiment efficiency can be improved, and used consumables and added toxic substances can be reduced; the method has high flexibility, one or more inhibitors or growth stimulants can be added to any culture layer, growth of the fungi can be interfered through the allelochemicals serving as signals, and the purpose of separation andpurification is achieved; the phage pollution of industrial fungi can be well separated, and a bacterial strain polluted by the phage is favorably purified; and the method is simple, convenient and easy to popularize, and has good fungus detection adaptability.

Description

technical field [0001] The invention relates to a biological detection technology, in particular to a method for separation and purification of high-contamination background fungi. Background technique [0002] As we all know, Trichoderma is difficult to purify and isolate due to the growth of many symbiotic fungi and bacteria in the habitat, and due to the competitive growth among fungi and spore scattering, the isolation effect is very poor and there are biosafety risks. Moreover, the traditional fungal culture experiment period is long, which greatly reduces the experimental efficiency. Trichoderma is one of the most widely used microbial species in industry. [0003] Because the background of the isolated Trichoderma is rich in various microorganisms, such as spores, actinomycetes, penicillium, aspergillus, phage, etc., the content is extremely high. Therefore, the liquid dilution method cannot ensure that the pollution of the background interfering bacteria is reduced....

AI Technical Summary

Problems solved by technology

[0003] Because the background of the isolated Trichoderma is rich in various microorganisms, such as spores, actinomycetes, penicillium, aspergillus, phage, etc., the content is extremely high. Therefore, the liquid dilution method cannot ensure that the pollution of the background interfering bacteria is reduced. Traditional The streaking method can not meet the separation purity requirements, therefore, adopting a better separation means and method is urgently needed in the fungal separation test