Nano-crystalline cellulose based 3D printing lactobacillus embedding material as well as preparation method and application thereof

A nanocellulose, 3D printing technology, applied in applications, dairy products, milk preparations, etc., can solve the problems of insufficient viscoelasticity of 3D printing ink, and achieve excellent 3D printing performance, efficient continuous cyclic fermentation, and effective embedding. Effect

Active Publication Date: 2019-12-10
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the viscoelasticity of 3D printing inks with only nanocellulose added is insufficient

Method used

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  • Nano-crystalline cellulose based 3D printing lactobacillus embedding material as well as preparation method and application thereof
  • Nano-crystalline cellulose based 3D printing lactobacillus embedding material as well as preparation method and application thereof
  • Nano-crystalline cellulose based 3D printing lactobacillus embedding material as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A preparation method of a nanocellulose-based 3D printed lactic acid bacteria embedding material, comprising the steps of:

[0046] (1) At room temperature, add 0.2g of sodium alginate powder to 20g of nanocellulose solution with a concentration of 0.03g / mL, mix well, then pour into the 3D printing syringe, and centrifuge to eliminate air bubbles. The speed is 2000rpm, and the centrifugation time is 1min to obtain the mixed solution;

[0047] (2) Add 0.01g of chitosan into 10mL of pure water, stir evenly to disperse the chitosan evenly in the water, then add 0.05mL of glacial acetic acid, mix well to completely dissolve the chitosan, and obtain a chitosan solution;

[0048] (3) Add 2g of lactic acid bacteria powder to the chitosan solution described in step (2), and mix evenly to obtain chitosan-lactic acid bacteria suspension;

[0049] (4) Use the mixture described in step (1) as the outer layer material, and the chitosan-lactic acid bacteria suspension described in s...

Embodiment 2

[0053] A preparation method of a nanocellulose-based 3D printed lactic acid bacteria embedding material, comprising the steps of:

[0054] (1) At room temperature, add 0.3g of sodium alginate powder to 20g of nanocellulose solution with a concentration of 0.04g / mL, mix well, then pour it into a 3D printing syringe, and centrifuge to eliminate air bubbles. The speed is 3000rpm, and the centrifugation time is 3min to obtain the mixed solution;

[0055] (2) Add 0.03g chitosan into 10mL pure water, stir evenly to disperse chitosan evenly in the water, then add 0.07mL glacial acetic acid, mix well, completely dissolve chitosan, and obtain chitosan solution;

[0056] (3) Add 1.5 g of lactic acid bacteria powder to the chitosan solution described in step (2), and mix evenly to obtain chitosan-lactic acid bacteria suspension;

[0057] (4) Use the mixture described in step (1) as the outer layer material, and the chitosan-lactic acid bacteria suspension described in step (3) as the in...

Embodiment 3

[0061] A preparation method of a nanocellulose-based 3D printed lactic acid bacteria embedding material, comprising the steps of:

[0062] (1) At room temperature, add 0.4g of sodium alginate powder to 20g of nanocellulose solution with a concentration of 0.05g / mL, mix well, then pour it into the 3D printing syringe, and centrifuge to eliminate air bubbles. The speed is 4000rpm, and the centrifugation time is 5min to obtain the mixed solution;

[0063] (2) Add 0.05g chitosan into 10mL pure water, stir evenly to disperse the chitosan evenly in the water, then add 0.1mL glacial acetic acid, mix well, completely dissolve the chitosan, and obtain a chitosan solution;

[0064] (3) Add 1.0 g of lactic acid bacteria powder to the chitosan solution described in step (2), and mix evenly to obtain chitosan-lactic acid bacteria suspension;

[0065] (4) Use the mixture described in step (1) as the outer layer material, and the chitosan-lactic acid bacteria suspension described in step (3...

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Abstract

The invention discloses a nano-crystalline cellulose based 3D printing lactobacillus embedding material as well as a preparation method and application thereof. The method comprises the following steps of adding sodium alginate in a nano-crystalline cellulose solution, uand performing uniform mixing and centrifuging to obtain a mixed solution; adding chitosan in water, performing uniform mixing, and adding glacial acetic acid to obtain a chitosan solution; adding lactobacillus powder in the chitosan solution, and performing uniform mixing to obtain a chitosan-lactobacillus bacterial suspension; taking the mixed solution as an outer material, and the chitosan-lactobacillus bacterial suspension as an inner material, and performing 3D printing to obtain a printing product; and spraying the surface of the printing product with a calcium chloride solution, and performing curing and cold drying to obtain the nano-crystalline cellulose based 3D printing lactobacillus embedding material. For the nano-crystalline cellulose based 3D printing lactobacillus embedding material, the lactobacillus is immobilized by taking the nano-crystalline cellulose and the chitosan as carriers, so that efficient continuous cyclic fermentation of yogurt can be realized, and the problems of difficulty in preservation of lactobacillus, low utilization rate and high cost can be solved.

Description

technical field [0001] The invention belongs to the field of food fermentation, and in particular relates to a nanocellulose-based 3D-printed lactic acid bacteria embedding material and a preparation method and application thereof. Background technique [0002] At present, the development momentum of my country's yogurt industry is very strong, and it is favored by consumers for its unique taste, flavor and high nutritional value. The lactic acid bacteria used in yogurt fermentation are a kind of human intestinal probiotics, which have been widely developed and applied in the fields of food and medicine. Due to the short storage period of wet lactic acid bacteria, vacuum freeze-drying technology is commonly used to make wet lactic acid bacteria into a dry state to prolong their storage time. However, the phase inversion process of lyophilization can cause microbial cell loss, death, or inactivation of certain enzyme proteins. For this reason, it is necessary to develop a g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10C12N11/04A23C9/123A23C9/13
CPCC12N11/14C12N11/10C12N11/04A23C9/1232A23C9/1307
Inventor 王小英周微吴正国
Owner SOUTH CHINA UNIV OF TECH
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