Suspension type liquid biological chip detection system

Abstract

The invention discloses a suspension type liquid biological chip detection system which comprises an upper cover plate, a front panel, a left panel, a right panel, a back plate, a liquid barrel fixingdevice, a light path system, a liquid path system, a circuit control system and a test tube picking and placing device, wherein a single laser is arranged in the light path system; according to the invention, nanocrystalline fluorescent microspheres are adopted; the emission peak width is relatively narrow; the particle size is controllable; optical coding can be conveniently carried out by usingdifferent wavelengths and microsphere particle sizes; classified fluorescence in the nanocrystalline fluorescent microspheres and labeled fluorescence of molecules to be detected in a sample are excited by using a single laser, a plurality of detection channels can be provided by the method, a larger number of information codes can be obtained, and the detection cost is greatly reduced by using asingle laser for excitation.

Description

technical field [0001] The invention relates to the field of medical instruments, in particular to a suspension type liquid biological chip detection system. Background technique [0002] Biochip (biochip) technology is a high-throughput biomolecular detection technology that integrates microelectronics, life science, computer science and photoelectrochemistry, and is a major revolution in the field of life science. The traditional form of biochip technology is also called microarray (microarray) technology. Its principle is to integrate biomolecules (DNA, RNA, polypeptide, protein, etc.) of known sequence on a solid surface to form a probe array. The detected biomolecules are hybridized with the above-mentioned probe array, and the detection of biomolecules is achieved by detecting the hybridization probes at the corresponding positions. Traditional biochip hybridization belongs to solid-liquid phase hybridization, and its discrete solid-liquid reaction environment and was...

AI Technical Summary

Problems solved by technology

[0004] At present, there are still technical problems to be solved in the existing commercial liquid biochip products: 1) Most of the fluorescent dyes used in the existing liquid biochip microspheres on the market are organic dyes. The emission peaks are easy to make the signals overlap and it is difficult to distinguish; 2) The photostability of organic fluorescent dyes is poor, and the ability to resist fluorescent bleaching is poor, which will affect its service life or storage period; 3) The classification fluorescence and sample on the microsphere probe The labeled fluorescence of the molecule to be detected must be excited by two different lasers, resulting in a high cost of the detection instrument; 4) This type of detection instrument has only two detection channels, and there are strict requirements on the size of the microspheres (5.6 μm Left and right), the restrictions on the setting of fluorescent signal parameters are large, which is not conducive to the future high-throughput and rapid clinical detection requirements and applications

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