Novel integrated pharmacology method and application thereof in treatment of breast cancer by astragalus membranaceus

A technology of pharmacology and breast cancer, applied in the field of integrated pharmacology, can solve problems such as response to intervention, research difficulties, and complex ingredients of traditional Chinese medicine, so as to avoid expensive costs, reduce false positive rates, and improve accuracy

Inactive Publication Date: 2019-12-10
刘存
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Disadvantages: 1) The composition of traditional Chinese medicine is complex, research is difficult, and its target information is even more difficult to summarize
2) Breast cancer is a complex and disordered system. Accumulated knowledge shows that this disease may not respond effectively to a single therapeutic intervention, and its pathogenesis and progression mechanisms as well as treatment strategies remain to be continuously studied
3) Clinical samples are limited and high-throughput sequencing is expensive
4) It is difficult to effectively correlate the multi-target characteristics of TCM disease intervention with complex disease systems

Method used

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  • Novel integrated pharmacology method and application thereof in treatment of breast cancer by astragalus membranaceus
  • Novel integrated pharmacology method and application thereof in treatment of breast cancer by astragalus membranaceus
  • Novel integrated pharmacology method and application thereof in treatment of breast cancer by astragalus membranaceus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Example 1: A new approach to integrative pharmacology

[0070] See attached Figure 1-10 , a new integrated pharmacology method of the present invention, the specific steps are as follows:

[0071] 1. Identification and analysis of differentially expressed genes

[0072] 1) Search and download disease-related gene chip microarray data from the Gene Expression Omnibus (GEO) database;

[0073] 2) Use the GEO online analysis tool GEO2R or the R language software package to analyze the microarray data of the downloaded chip, and set the absolute value of the differential change multiple greater than 1 and the statistical P value less than 0.05 as the screening criteria for differentially expressed genes;

[0074] 3) Use the online Venn diagram software to obtain the common genes of differentially expressed genes in diseases for further analysis;

[0075] 4) Use the online Morpheus software to visually edit the final disease differentially expressed gene data to obtain a ...

Embodiment 2

[0100] Example 2: Application of a new integrative pharmacology method in the treatment of breast cancer by astragalus

[0101] See attached Figure 1-10 , the application of a new integrated pharmacology method of the present invention in the treatment of breast cancer by Radix Astragali, the specific steps are as follows:

[0102] 1. Identification and analysis of differentially expressed genes

[0103] 1) Search and download breast cancer-related gene chip microarray data from the Gene Expression Omnibus (GEO) database, use breast cancer, normal tissue samples and genes as keywords for screening, and finally obtain 3 sets of chips for exploration (GSE38959, GSE65194 and GSE76275), such as Figure 8 shown;

[0104] 2) Use the GEO online analysis tool GEO2R or the R language software package to analyze the microarray data of the downloaded chip, set the absolute value of the differential change multiple greater than 1 and the statistical P value less than 0.05 as the scree...

Embodiment 3

[0125] Example 3 Verification of the application of a new integrated pharmacology method in the treatment of breast cancer by astragalus

[0126] See attached Figure 1-10 , on the basis of Example 2, the results obtained in Example 2 are verified.

[0127] The verification includes the following steps:

[0128] 1: Cell culture

[0129] 1) Purchase human breast cancer cell lines MCF-7 and MDA-MB-231;

[0130] 2) Resuscitate and subculture in the culture medium, and culture at 37°C and 5% CO 2 in the cell culture incubator.

[0131] 2: Cell Viability Assay

[0132] 1) MCF-7 and MDA-MB-231 cells were seeded into 96-well microplates (5×10 3 cells / well) and allowed to adhere overnight for a total of three replicate wells in each group;

[0133] 2) Remove the medium the next day and add fresh medium containing astragalus polysaccharide (0, 0.25, 0.5, 1 or 2 mg / ml);

[0134] 3) Add 10 μl of Cell Count-8 Reagent to each well at the indicated time points (1, 2, 3 and 4 days af...

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Abstract

The invention provides a novel integrated pharmacology method. The method comprises the steps of differential expression gene identification and analysis, traditional Chinese medicine component database construction and ADME screening, identification of related compound targets, introduction and annotation of the compound targets, protein interaction network construction and analysis and moleculardynamics simulation and target fishing. The invention also provides an application of the novel integrated pharmacology method in the treatment of breast cancer by astragalus membranaceus. Multi-target effective components and targets are comprehensively modeled on the biological path and interaction network level, and the complex and multi-level interaction network is analyzed, so the interaction between the medicine and the specific node or module can be discovered, and a new target can be discovered; the detected target is more comprehensive and high in accuracy, and the multi-target characteristic of the traditional Chinese medicine intervention disease is effectively associated with the complex disease system.

Description

technical field [0001] The invention relates to the field of integrated pharmacology, in particular to a new integrated pharmacology method and its application in treating breast cancer with astragalus. Background technique [0002] Breast cancer (BC), one of the most common cancers, remains a serious health threat for women, accounting for approximately 30% of cancer cases in women each year. Although new treatment strategies have gradually improved the survival rate of breast cancer patients, many of them still face recurrence problems and the long-term mortality rate remains high. In addition, many treatments are associated with drug resistance and serious side effects, which can affect patients' quality of life. Therefore, it is necessary to find new therapeutic agents or emerging targets. [0003] As arsenic has made a major breakthrough in the field of leukemia treatment, the unique antitumor activity of many natural products has attracted people's attention. Astrag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16C10/00G16C20/30G16C20/80C12Q1/6886
CPCC12Q1/6886C12Q2600/136C12Q2600/158
Inventor 刘瑞娟刘存孙长岗庄静刘丽娟周超高春迪李华瑶
Owner 刘存
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