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Extraction method of flammulina velutipes polysaccharides

A technology of Flammulina velutipes polysaccharide and extraction method, which is applied in the field of plant polysaccharide extraction, can solve the problems of low extraction rate, reduced purity of Flammulina velutipes polysaccharide, and reduced biological activity of Flammulina velutipes polysaccharide, etc., and achieves the effects of improving extraction rate and improving biological activity.

Inactive Publication Date: 2019-12-13
沈阳翔源科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] This method can extract Flammulina velutipes polysaccharide, but in this method, the extraction yield of Flammulina velutipes is only 1.1254%, the extraction rate is very low, and the extracted Flammulina velutipes polysaccharide contains more protein impurities, which reduces the purity of Flammulina velutipes polysaccharides, and then Reduced the biological activity of the extracted Flammulina velutipes polysaccharide

Method used

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  • Extraction method of flammulina velutipes polysaccharides
  • Extraction method of flammulina velutipes polysaccharides
  • Extraction method of flammulina velutipes polysaccharides

Examples

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Embodiment 1

[0030] 1) Wash the fruiting body of Flammulina velutipes, dry it and grind it into powder, add the dry powder of Flammulina velutipes into distilled water, extract in a water bath for 5 hours, place the extract in a centrifuge at 3000r / min for 10min, take the centrifuged supernatant and put it under vacuum at 50°C Concentrate to 1 / 4 of the original volume;

[0031] 2) Refining extraction: Dialyze the concentrated solution after vacuum concentration in distilled water at 4°C for 24-48h, place it in a centrifuge at 3000r / min for 10min, take the supernatant, and freeze-dry to obtain Flammulina velutipes polysaccharide;

[0032] 3) Storage: store at -20°C;

[0033] Under the condition that the ratio of solid to liquid and the extraction time were kept constant, the polysaccharide yields were tested at the extraction temperatures of 50, 60, 70, 80, 90°C and 100°C, and the results were as follows: figure 1 As shown, under the condition that the extraction time is 5h and the solid-l...

Embodiment 2

[0042]1) Wash the fruiting body of Flammulina velutipes, dry it and grind it into powder, add the dry powder of Flammulina velutipes into distilled water, extract in a water bath for 5 hours, place the extract in a centrifuge at 3000r / min for 10min, take the centrifuged supernatant and put it under vacuum at 50°C Concentrate to 1 / 4 of the original volume;

[0043] 2) Refining extraction: Dialyze the concentrated solution after vacuum concentration in distilled water at 4°C for 24-48h, place it in a centrifuge at 3000r / min for 10min, take the supernatant, and freeze-dry to obtain Flammulina velutipes polysaccharide;

[0044] 3) Redissolve the preserved Flammulina velutipes polysaccharides with hot water, and perform high-voltage pulse electric field treatment. The molecular polarization of the electric field induces more charged polar groups to be exposed, and induces charged molecules to produce repeated violent movements, destroying the residual protein in Flammulina velutipes...

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Abstract

The invention relates to the technical field of plant polysaccharide extraction, in particular to an extraction method of xtraction method of flammulina velutipes polysaccharides. The extraction method comprises the following steps: cleaning sporocarp of flammulina velutipes, drying in the sun, grinding into powder, adding obtained flammulina velutipes dry powder into distilled water, extracting in a water bath, centrifuging an obtained extracted solution, and carrying out vacuum concentration on an obtained centrifuged supernate at 50 DEG C to 1 / 4 of the original volume. The extraction rate of the flammulina velutipes polysaccharides obtained by the method is up to 8.21%; the extraction rate of the flammulina velutipes polysaccharides is greatly improved; according to the extraction method, Saveg liquid and a sugar liquid are mixed and uniformly shaken, denatured proteins are filtered out, operation is repeated for multiple times, the content of proteins in the flammulina velutipes polysaccharides is basically zero at the moment, the high-purity flammulina velutipes polysaccharides without proteins can be obtained, and the biological activity of the flammulina velutipes polysaccharides is improved.

Description

technical field [0001] The invention relates to the technical field of extracting plant polysaccharides, in particular to a method for extracting polysaccharides from Flammulina velutipes. Background technique [0002] Flammulina velutipes another name hair handle money mushroom, chrysanthemum, shiitake mushroom, port mushroom and structure bacteria etc. Branch and Pyromyces genus (Yu Rongli, Qin Xusheng, Song Fengju. Overview of Flammulina velutipes research [J]. Journal of Edible Fungi, 2004, 11(4):63-68). Named for its golden and tender stalks like golden needles, it is one of the precious edible fungi. At present, researchers have been able to produce Flammulina velutipes polysaccharides from Flammulina velutipes. Numerous studies have shown that Flammulina velutipes polysaccharides have good antioxidant properties, lower blood lipids, improve Biological activities such as immunity and anti-aging have high economic benefits, so it is necessary to extract the polysacchar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 任清张莎莎孙静王晶淼邓巍左桥
Owner 沈阳翔源科技有限公司
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