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Horseradish peroxidase @ MOF composite catalyst and preparation method thereof

A technology of horseradish peroxidase and composite catalyst, which is applied to biochemical equipment and methods, oxidoreductases, enzymes and other directions, can solve the problems of high environmental requirements, secondary pollution, low stability and the like, and achieves a simple preparation method. , easy recovery, mild operating conditions

Active Publication Date: 2019-12-20
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the disadvantages of low enzyme stability in the catalytic process, high environmental requirements, and easy to cause secondary pollution in the prior art, the purpose of the present invention is to provide a horseradish peroxidase@MOF composite catalyst and its preparation Method and application, by first modifying the horseradish peroxidase, and then preparing the MOF shell material, and then using the weak interaction between the horseradish peroxidase and the MOF, allowing the enzyme to enter the pores of the MOF A highly efficient horseradish peroxidase@MOF composite catalyst was formed

Method used

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  • Horseradish peroxidase @ MOF composite catalyst and preparation method thereof
  • Horseradish peroxidase @ MOF composite catalyst and preparation method thereof
  • Horseradish peroxidase @ MOF composite catalyst and preparation method thereof

Examples

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Effect test

Embodiment 1

[0035] Embodiment 1: Preparation of horseradish peroxidase@FeBTC composite catalyst

[0036] First, dissolve 0.4167g of dimethyl sulfoxide in 10mL of deionized water, add 0.003g of phthalic anhydride, and mix well; take 0.004g of horseradish catalase and dissolve it in 4mL of deionized water, add 0.3mL of the above Diformic anhydride solution was stirred at 4 °C for 1 h. Centrifuge and wash with water to remove excess modifier. Then, 0.002 g of the pre-prepared FeBTC shell MOF material was added to 4 mL of deionized water to make it uniformly dispersed by ultrasound, and then the modified horseradish peroxidase was added therein, and stirred at 4 °C for 2 h. After the reaction was completed, it was centrifuged and washed three times with deionized water, and then dried at 20° C. to obtain the horseradish peroxidase@FeBTC composite catalyst.

[0037]The horseradish peroxidase@FeBTC composite catalyst was tested for o-phenylenediamine catalysis, and the conversion rate of o-ph...

Embodiment 2

[0038] Example 2: Preparation of horseradish peroxidase@CuBTC composite catalyst

[0039] First, dissolve 0.4167g of dimethyl sulfoxide in 10mL of deionized water, add 0.003g of phthalic anhydride, and mix well; take 0.004g of horseradish catalase and dissolve it in 4mL of deionized water, add 0.3mL of the above Diformic anhydride solution was stirred at 4 °C for 1 h. Centrifuge and wash with water to remove excess modifier. Then, 0.002 g of the pre-prepared CuBTC shell MOF material was added to 4 mL of deionized water to make it uniformly dispersed by ultrasonic, and then the modified horseradish peroxidase was added, and stirred at 4 °C for 2 h. After the reaction was completed, it was centrifuged and washed three times with deionized water, and then dried at 20° C. to obtain the horseradish peroxidase@CuBTC composite catalyst.

[0040] The horseradish peroxidase@CuBTC composite catalyst was tested for o-phenylenediamine catalysis, and the conversion rate of o-phenylenedia...

Embodiment 3

[0041] Embodiment 3: Preparation of horseradish peroxidase@CuBDC composite catalyst

[0042] First, dissolve 0.4167g of dimethyl sulfoxide in 10mL of deionized water, add 0.003g of phthalic anhydride, and mix well; take 0.004g of horseradish catalase and dissolve it in 4mL of deionized water, add 0.3mL of the above Diformic anhydride solution was stirred at 4 °C for 1 h. Centrifuge and wash with water to remove excess modifier. Then, 0.002 g of the pre-prepared CuBDC shell MOF material was added to 4 mL of deionized water to make it uniformly dispersed by ultrasonic, and then the modified horseradish peroxidase was added, and stirred at 4 °C for 2 h. After the reaction was completed, it was centrifuged and washed three times with deionized water, and then dried at 20° C. to obtain the horseradish peroxidase@CuBDC composite catalyst.

[0043] The horseradish peroxidase@CuBDC composite catalyst was tested and analyzed for o-phenylenediamine catalysis experiments. The conversio...

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Abstract

The present invention discloses a horseradish peroxidase @ MOF composite catalyst and a preparation method thereof. The preparation method comprises the following steps: dissolving dimethyl sulfoxidein deionized water to obtain a dimethyl sulfoxide aqueous solution, dissolving horseradish peroxidase in deionized water to obtain a horseradish peroxidase aqueous solution, adding phthalic anhydridein the dimethyl sulfoxide aqueous solution to obtain a mixed solution, adding the mixed solution into the horseradish peroxidase aqueous solution after ultrasonic uniform mixing, conducting stirring at 0-4 DEG C for 1-3 h, and centrifuging and washing the product to obtain a modified horseradish peroxidase; dispersing MOF shell materials into deionized water to obtain a dispersion liquid of the MOF shell materials, then adding the obtained modified horseradish peroxidase into the dispersion liquid of the MOF shell materials, and conducting stirring for reaction to obtain the horseradish peroxidase @ MOF composite catalyst. The horseradish peroxidase @ MOF composite catalyst can be applied to catalytic conversion of o-phenylenediamine.

Description

[0001] (1) Technical field [0002] The invention relates to a simple and efficient horseradish peroxidase@MOF composite catalyst with mild conditions and a preparation method thereof, belonging to the technical field of biocatalytic materials. [0003] (2) Background technology [0004] Enzymes, one of the most important biomacromolecules in life, far exceed the ability of artificial catalysts to efficiently catalyze life-sustaining biotransformations, making it attractive to apply in vivo enzymatic transformations to industrial processes. Applications are hampered by enzyme fragility, such as low thermal stability, narrow pH optimum range, low tolerance to most organic solvents and many metal ions, etc. In addition, the enzyme itself is a source of contamination in the desired product, leading to unavoidable purification and separation steps that easily form secondary pollution. "Immobilized enzyme" refers to an enzyme that is physically restricted or located in a specific s...

Claims

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Application Information

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IPC IPC(8): C12N11/14
CPCC12N9/0065C12N11/14C12Y111/01007
Inventor 张国亮李畅
Owner ZHEJIANG UNIV OF TECH
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