Preparation method and application of endometrial stem cell preparation
A stem cell preparation and endometrium technology, which is applied in the fields of regenerative medicine and biology, can solve the problems that products cannot be stored and transported for a long time, and achieve the effects of preventing and treating premature ovarian failure, convenient transportation and good curative effect.
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Embodiment 1
[0038] Example 1: Preparation and identification of endometrial stem cells
[0039] 1. Tissue reception: After consultation and consent, sign the "Patient Information and Consent Form" with the patient, collect postoperative clinical samples (endometrial tissue) from the patient, place them in normal saline, seal them, and store them as soon as possible within 1 hour sent to the laboratory;
[0040] 2. Tissue processing: In the ultra-clean workbench, take out the received clinical endometrial tissue, put it into a petri dish, spread the tissue, and measure the length and width of the tissue. After the measurement, use sterile scissors and tweezers to cut into 1mm 3 The small pieces were washed three times with PBS;
[0041] 3. Tissue digestion: move the tissue block into a 50mL centrifuge tube, add 2 times the volume of PBS solution containing 300mg / mL collagenase III, tighten the tube mouth, shake and digest in a 37°C incubator for 40min;
[0042] 4. Obtaining primary cell...
Embodiment 2
[0049] Example 2: Prevention and treatment of premature ovarian failure with endometrial stem cell preparation
[0050] 1. Experimental animal preparation: select 60 wistar female rats aged 2-3 months, with a body weight of 180-200g, and have a normal estrous cycle. Model control group, and the remaining 40 rats were modeled.
[0051] 2. Establishment of premature ovarian failure model in rats: Give rats tripterygium glycosides tablet suspension 50mg / kg daily, gavage continuously for 14 days, take light smears of vaginal secretions with cotton swab at 8:00 every day, after Pap staining, observe Changes in exfoliated cells of the vagina. After gavage, blood was collected from the tail vein of the rats, and the levels of estradiol and follicle stimulating hormone in the blood were detected, and compared with those of the modeling control group. When the continuous estrous period was observed for 10 consecutive days, and the level of estradiol in serum was significantly lower t...
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